C57BL/6JCya-Eml2em1flox/Cya
Common Name:
Eml2-flox
Product ID:
S-CKO-18747
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Eml2-flox
Strain ID
CKOCMP-72205-Eml2-B6J-VB
Gene Name
Product ID
S-CKO-18747
Gene Alias
1600029N02Rik; EMAP-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Eml2em1flox/Cya mice (Catalog S-CKO-18747) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000117338
NCBI RefSeq
NM_001347582
Target Region
Exon 8~9
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Eml2, short for echinoderm microtubule-associated protein-like 2, is a gene with significant functions. It preferentially interacts with tyrosinated (Y -) microtubules through a WD40-repeat-based Y-microtubule interaction motif, influencing microtubule dynamics to increase stability [1]. In plants like Arabidopsis thaliana, EML2 forms a complex with GTE4, recognizing histone acetylation and H3K4 trimethylation to facilitate gene transcription [3].
In human diseases, EML2 has been studied in colon cancer. High EML2 expression in colon cancer tissues is associated with poor prognosis and altered immune cell infiltration, suggesting its potential as a prognostic biomarker [2]. Additionally, in familial nasopharyngeal carcinoma, EML2 was found to be enriched, along with other genes, in both NPC and other Epstein-Barr virus-associated cancers [4]. DNA methylation of EML2 in sporadic pituitary adenomas is inversely related to its transcript expression, indicating its role in tumor-related epigenetic regulation [5].
In summary, Eml2 is involved in microtubule regulation and gene transcription in different organisms. In human cancers such as colon cancer and nasopharyngeal carcinoma, as well as in pituitary adenomas, Eml2 shows potential associations with disease prognosis and epigenetic regulation. Research on Eml2 provides insights into disease mechanisms and possible biomarker identification.
References:
1. Hotta, Takashi, McAlear, Thomas S, Yue, Yang, Bechstedt, Susanne, Ohi, Ryoma. 2022. EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules. In Current biology : CB, 32, 3898-3910.e14. doi:10.1016/j.cub.2022.07.027. https://pubmed.ncbi.nlm.nih.gov/35963242/
2. Sun, Yanjun, Han, Lin, Sun, Dengqun. 2024. Comprehensive analysis of EML2 as a prognostic biomarker in colon cancer. In International journal of clinical and experimental pathology, 17, 1-12. doi:. https://pubmed.ncbi.nlm.nih.gov/38322176/
3. Qian, Feng, Zhao, Qiang-Qiang, Zhou, Jin-Xing, Chen, She, He, Xin-Jian. . The GTE4-EML chromatin reader complex concurrently recognizes histone acetylation and H3K4 trimethylation in Arabidopsis. In The Plant cell, 37, . doi:10.1093/plcell/koae330. https://pubmed.ncbi.nlm.nih.gov/39692581/
4. Wang, Tong-Min, He, Yong-Qiao, Xue, Wen-Qiong, Lung, Maria Li, Jia, Wei-Hua. . Whole-Exome Sequencing Study of Familial Nasopharyngeal Carcinoma and Its Implication for Identifying High-Risk Individuals. In Journal of the National Cancer Institute, 114, 1689-1697. doi:10.1093/jnci/djac177. https://pubmed.ncbi.nlm.nih.gov/36066420/
5. Duong, Cuong V, Emes, Richard D, Wessely, Frank, Clayton, Richard N, Farrell, William E. 2012. Quantitative, genome-wide analysis of the DNA methylome in sporadic pituitary adenomas. In Endocrine-related cancer, 19, 805-16. doi:10.1530/ERC-12-0251. https://pubmed.ncbi.nlm.nih.gov/23045325/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen