C57BL/6JCya-Tmem106bem1flox/Cya
Common Name:
Tmem106b-flox
Product ID:
S-CKO-18755
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tmem106b-flox
Strain ID
CKOCMP-71900-Tmem106b-B6J-VB
Gene Name
Product ID
S-CKO-18755
Gene Alias
2310036D22Rik; 5830455K21Rik; 6430519M21Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem106bem1flox/Cya mice (Catalog S-CKO-18755) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031556
NCBI RefSeq
NM_027992
Target Region
Exon 4~5
Size of Effective Region
~4.0 kb
Detailed Document
Overview of Gene Research
TMEM106B, a lysosomal transmembrane protein, is involved in regulating lysosomal function and myelin lipid metabolism [3,4]. It may also play a role in ACE2-independent SARS-CoV-2 cell entry [2]. Mutations in TMEM106B are risk factors for diverse neurodegenerative diseases [1].
In mouse models, TMEM106B deficiency significantly decreases levels of myelin lipids, galactosylceramide and sulfatide, and increases galactosylceramidase activity, suggesting its role in regulating myelin lipid metabolism [4]. However, full deletion or partial reduction of TMEM106B in mouse and iPSC-derived human cell models of GRN deficiency did not reverse disease-associated phenotypes, indicating that lowering TMEM106B levels may not be a viable therapeutic strategy for treating FTD-GRN [5].
In conclusion, TMEM106B is crucial for lysosomal function and myelin lipid metabolism. Mouse models have provided insights into its functions and potential implications in neurodegenerative diseases, such as frontotemporal dementia. Although reducing TMEM106B levels may not be a therapeutic solution for FTD-GRN, understanding its role can still contribute to the understanding of neurodegenerative disease mechanisms [1,3,4,5].
References:
1. Jiao, Hai-Shan, Yuan, Peng, Yu, Jin-Tai. 2023. TMEM106B aggregation in neurodegenerative diseases: linking genetics to function. In Molecular neurodegeneration, 18, 54. doi:10.1186/s13024-023-00644-1. https://pubmed.ncbi.nlm.nih.gov/37563705/
2. Baggen, Jim, Jacquemyn, Maarten, Persoons, Leentje, Cherepanov, Peter, Daelemans, Dirk. 2023. TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entry. In Cell, 186, 3427-3442.e22. doi:10.1016/j.cell.2023.06.005. https://pubmed.ncbi.nlm.nih.gov/37421949/
3. Zhu, Min, Zhang, Guoxin, Meng, Lanxia, Fang, Xin, Zhang, Zhentao. 2024. Physiological and pathological functions of TMEM106B in neurodegenerative diseases. In Cellular and molecular life sciences : CMLS, 81, 209. doi:10.1007/s00018-024-05241-z. https://pubmed.ncbi.nlm.nih.gov/38710967/
4. Takahashi, Hideyuki, Perez-Canamas, Azucena, Lee, Chris W, Han, Xianlin, Strittmatter, Stephen M. 2024. Lysosomal TMEM106B interacts with galactosylceramidase to regulate myelin lipid metabolism. In Communications biology, 7, 1088. doi:10.1038/s42003-024-06810-5. https://pubmed.ncbi.nlm.nih.gov/39237682/
5. Dominguez, Sara L, Laufer, Benjamin I, Ghosh, Arundhati Sengupta, Meilandt, William J, Easton, Amy. 2023. TMEM106B reduction does not rescue GRN deficiency in iPSC-derived human microglia and mouse models. In iScience, 26, 108362. doi:10.1016/j.isci.2023.108362. https://pubmed.ncbi.nlm.nih.gov/37965143/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen