C57BL/6JCya-Tjp2em1flox/Cya
Common Name:
Tjp2-flox
Product ID:
S-CKO-18757
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tjp2-flox
Strain ID
CKOCMP-21873-Tjp2-B6J-VB
Gene Name
Product ID
S-CKO-18757
Gene Alias
ZO-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tjp2em1flox/Cya mice (Catalog S-CKO-18757) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000099558
NCBI RefSeq
NM_011597
Target Region
Exon 4~5
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Tjp2, also known as ZO-2, encodes a tight junction protein. Liver tight junctions established by Tjp2 isolate bile from the blood circulation. Tjp2 is involved in maintaining the normal cortical distribution of radixin, canalicular volume regulation, and microvilli density in the liver [1].
In mice with liver-specific inactivation of Tjp2 (Tjp2 cKO mice), there are lower Cldn1 protein levels, minor TJ changes, dilated canaliculi, lower microvilli density, and abnormal radixin and BSEP distribution. These mice present with mild progressive cholestasis, lower expression of bile acid transporter Abcb11/Bsep and detoxification enzyme Cyp2b10. They are more susceptible to cholic acid-induced liver injury. Hepatocyte-specific Tjp2 inactivation can lead to hepatocyte to cholangiocyte transdifferentiation in response to a DDC-supplemented diet, which requires Yap and Wwtr1/Taz whose protein expression is upregulated in hepatocytes lacking Tjp2 [1,2].
In conclusion, Tjp2 is essential for maintaining normal liver structure and function related to bile acid transport and the integrity of tight junctions. Tjp2 cKO mouse models have revealed its role in cholestatic liver diseases, such as its involvement in the pathophysiology of progressive cholestasis and the transdifferentiation process in the liver [1,2].
References:
1. Xu, Jianliang, Kausalya, P Jaya, Van Hul, Noémi, Kaldis, Philipp, Hunziker, Walter. 2021. Protective Functions of ZO-2/Tjp2 Expressed in Hepatocytes and Cholangiocytes Against Liver Injury and Cholestasis. In Gastroenterology, 160, 2103-2118. doi:10.1053/j.gastro.2021.01.027. https://pubmed.ncbi.nlm.nih.gov/33465371/
2. Xu, Jianliang, Kausalya, P Jaya, Ong, Alicia Ghia Min, Mohamed Ali, Safiah, Hunziker, Walter. 2022. ZO-2/Tjp2 suppresses Yap and Wwtr1/Taz-mediated hepatocyte to cholangiocyte transdifferentiation in the mouse liver. In NPJ Regenerative medicine, 7, 55. doi:10.1038/s41536-022-00251-6. https://pubmed.ncbi.nlm.nih.gov/36151109/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen