C57BL/6JCya-Zfp503em1flox/Cya
Common Name:
Zfp503-flox
Product ID:
S-CKO-18760
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zfp503-flox
Strain ID
CKOCMP-218820-Zfp503-B6J-VB
Gene Name
Product ID
S-CKO-18760
Gene Alias
B830002A16Rik; Nolz-1; Nolz1; Znf503; Zpo2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp503em1flox/Cya mice (Catalog S-CKO-18760) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000043409
NCBI RefSeq
NM_145459
Target Region
Exon 3
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Zfp503, also known as Nlz2 or Nolz-1, is a zinc-finger containing transcription factor. It is involved in multiple biological processes such as cell fate determination, organ development, and is regulated by various signaling pathways like hedgehog and BMP signaling [4]. Genetic models, especially KO mouse models, have been crucial in understanding its functions.
In eye development, Zfp503 KO mice exhibit uveal coloboma due to failure of optic fissure closure and abnormal RPE differentiation. Altered mRNA levels and expression patterns of key eye-development transcription factors were observed, leading to reduced melanin pigmentation and RPE differentiation into a neural retina-like lineage [1].
In striatal development, D1 MSNs in Zfp503-deficient mice switch to D2 MSNs, and Zfp503 affects progenitor cell differentiation in the lateral ganglionic eminence by regulating Sp8/9 expression [2].
In the developing brain, CD93 activates Zfp503 transcription through β-Catenin, and Zfp503, as a transcriptional repressor, inhibits astrogenesis by binding to the Gfap promoter with Grg5 [3].
In conclusion, Zfp503 plays essential roles in eye development, neural cell fate determination, and astrogenesis. Studies using KO mouse models have revealed its significance in these processes, providing insights into related congenital eye malformations and neural development-associated disorders [1,2,3].
References：
1. Boobalan, Elangovan, Thompson, Amy H, Alur, Ramakrishna P, Bharti, Kapil, Brooks, Brian P. . Zfp503/Nlz2 Is Required for RPE Differentiation and Optic Fissure Closure. In Investigative ophthalmology & visual science, 63, 5. doi:10.1167/iovs.63.12.5. https://pubmed.ncbi.nlm.nih.gov/36326727/
2. Shang, Zicong, Yang, Lin, Wang, Ziwu, Li, Zhenmeiyu, Zhang, Zhuangzhi. 2022. The transcription factor Zfp503 promotes the D1 MSN identity and represses the D2 MSN identity. In Frontiers in cell and developmental biology, 10, 948331. doi:10.3389/fcell.2022.948331. https://pubmed.ncbi.nlm.nih.gov/36081908/
3. Liang, Qingli, Su, Libo, Zhang, Dongming, Jiao, Jianwei. 2020. CD93 negatively regulates astrogenesis in response to MMRN2 through the transcriptional repressor ZFP503 in the developing brain. In Proceedings of the National Academy of Sciences of the United States of America, 117, 9413-9422. doi:10.1073/pnas.1922713117. https://pubmed.ncbi.nlm.nih.gov/32291340/
4. McGlinn, Edwina, Richman, Joy M, Metzis, Vicki, Wainwright, Brandon J, Wicking, Carol. . Expression of the NET family member Zfp503 is regulated by hedgehog and BMP signaling in the limb. In Developmental dynamics : an official publication of the American Association of Anatomists, 237, 1172-82. doi:10.1002/dvdy.21508. https://pubmed.ncbi.nlm.nih.gov/18351672/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen