C57BL/6JCya-Sfswapem1flox/Cya
Common Name:
Sfswap-flox
Product ID:
S-CKO-18775
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sfswap-flox
Strain ID
CKOCMP-231769-Sfswap-B6J-VB
Gene Name
Product ID
S-CKO-18775
Gene Alias
1190005N23Rik; 6330437E22Rik; SWAP; Sfrs8; Srsf8
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sfswapem1flox/Cya mice (Catalog S-CKO-18775) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000053737
NCBI RefSeq
NM_172276
Target Region
Exon 2~4
Size of Effective Region
~4.0 kb
Detailed Document
Overview of Gene Research
Sfswap, a poorly characterized splicing factor, is involved in multiple biological processes. It plays a role in regulating pre-mRNA splicing, especially as a negative regulator of global pre-mRNA splicing and a positive regulator of intron retention. It is also associated with the O-GlcNAcylation pathway, where it regulates OGT intron detention. Additionally, it is implicated in the development of the inner ear [1,2,3].
In mouse models, homozygous Sfswap mutants display vestibular and cochlear defects consistent with disrupted Notch signaling. These mutants show hyperactivity, circling behavior, and significantly impaired hearing. The cochlea of newborn Sfswap mutant mice has a reduction in outer hair cells and supporting cells, along with ectopic inner hair cells. The phenotype is similar to that of hypomorphic alleles of the Notch ligand Jagged1 (Jag1), and Jag1;Sfswap compound mutants have more severe inner ear defects, indicating a genetic interaction between Sfswap and Jag1. Also, expression of genes involved in Notch signaling in the inner ear is reduced in Sfswap mutants [2].
In conclusion, Sfswap is an important regulator of pre-mRNA splicing and has a significant impact on inner ear development. The study of Sfswap knockout mouse models has revealed its role in disrupting Notch signaling in the inner ear, contributing to our understanding of inner ear-related diseases.
References:
1. Govindan, Ashwin, Conrad, Nicholas K. 2025. SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.10.14.618288. https://pubmed.ncbi.nlm.nih.gov/39464125/
2. Moayedi, Yalda, Basch, Martin L, Pacheco, Natasha L, Mardon, Graeme, Groves, Andrew K. 2014. The candidate splicing factor Sfswap regulates growth and patterning of inner ear sensory organs. In PLoS genetics, 10, e1004055. doi:10.1371/journal.pgen.1004055. https://pubmed.ncbi.nlm.nih.gov/24391519/
3. Govindan, Ashwin, Conrad, Nicholas K. 2025. SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing. In eLife, 13, . doi:10.7554/eLife.104439. https://pubmed.ncbi.nlm.nih.gov/40265571/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen