C57BL/6JCya-Pax7em1flox/Cya
Common Name:
Pax7-flox
Product ID:
S-CKO-18778
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Pax7-flox
Strain ID
CKOCMP-18509-Pax7-B6J-VB
Gene Name
Product ID
S-CKO-18778
Gene Alias
Pax-7
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pax7em1flox/Cya mice (Catalog S-CKO-18778) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030508
NCBI RefSeq
NM_011039
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Pax7, a transcription factor, is a key regulator in myogenesis [3,4,6]. It is commonly used as an indicator in muscle research [3]. Pax7 is closely associated with the function of muscle stem cells, especially satellite cells, which are crucial for skeletal muscle maintenance, repair, and regeneration [1,2,3,4,5,6,8]. Pax7 may also be involved in the self-renewal of spermatogonial stem cells in dairy goats [7]. Pax7 reporter mouse models have been developed to study satellite cell-mediated events [8].
In human skeletal muscle, an acute bout of exercise affects Pax7+ satellite cells. Resistance exercise leads to a large increase in Pax7+ cell content in skeletal muscles 24 h after exercise, with cells increasing to ~50% above pre-exercise level 24-72 h after resistance exercise. In adults over 50 years old, resistance exercise has a large effect on increasing Pax7+ cells in exercised muscle, while in younger adults, it has a medium effect [1]. In vitro, human skeletal muscle organoids can generate sustainable uncommitted PAX7 myogenic progenitors, which can regenerate muscle when transplanted into adult mice [2].
In conclusion, Pax7 is essential for myogenesis, especially in post-natal growth, muscle regeneration, and the function of satellite cells. Research on Pax7, including through the use of mouse models, provides insights into muscle-related processes and may potentially contribute to understanding and treating muscle-related disorders such as muscular dystrophies [3].
References:
1. Dewi, Luthfia, Lin, Yin-Chou, Nicholls, Andrew, Huang, Chih-Yang, Kuo, Chia-Hua. 2022. Pax7+ Satellite Cells in Human Skeletal Muscle After Exercise: A Systematic Review and Meta-analysis. In Sports medicine (Auckland, N.Z.), 53, 457-480. doi:10.1007/s40279-022-01767-z. https://pubmed.ncbi.nlm.nih.gov/36266373/
2. Mavrommatis, Lampros, Jeong, Hyun-Woo, Kindler, Urs, Brand-Saberi, Beate, Zaehres, Holm. 2023. Human skeletal muscle organoids model fetal myogenesis and sustain uncommitted PAX7 myogenic progenitors. In eLife, 12, . doi:10.7554/eLife.87081. https://pubmed.ncbi.nlm.nih.gov/37963071/
3. Rahman, Nor Idayu A, Lam, Chung Liang, Sulaiman, Nadiah, Ariffin, Shahrul Hisham Zainal, Yazid, Muhammad Dain. 2023. PAX7, a Key for Myogenesis Modulation in Muscular Dystrophies through Multiple Signaling Pathways: A Systematic Review. In International journal of molecular sciences, 24, . doi:10.3390/ijms241713051. https://pubmed.ncbi.nlm.nih.gov/37685856/
4. Azhar, Muhamad, Wardhani, Bantari Wisynu Kusuma, Renesteen, Editha. 2022. The regenerative potential of Pax3/Pax7 on skeletal muscle injury. In Journal, genetic engineering & biotechnology, 20, 143. doi:10.1186/s43141-022-00429-x. https://pubmed.ncbi.nlm.nih.gov/36251225/
5. Brack, Andrew S. 2014. Pax7 is back. In Skeletal muscle, 4, 24. doi:10.1186/s13395-014-0024-4. https://pubmed.ncbi.nlm.nih.gov/25546147/
6. Buckingham, Margaret, Relaix, Frédéric. 2015. PAX3 and PAX7 as upstream regulators of myogenesis. In Seminars in cell & developmental biology, 44, 115-25. doi:10.1016/j.semcdb.2015.09.017. https://pubmed.ncbi.nlm.nih.gov/26424495/
7. Du, Xiaomin, Wu, Siyu, Wei, Yudong, Li, Guangpeng, Hua, Jinlian. 2020. PAX7 promotes CD49f-positive dairy goat spermatogonial stem cells' self-renewal. In Journal of cellular physiology, 236, 1481-1493. doi:10.1002/jcp.29954. https://pubmed.ncbi.nlm.nih.gov/32692417/
8. Ortuste Quiroga, Huascar Pedro, Fujimaki, Shin, Ono, Yusuke. 2023. Pax7 reporter mouse models: a pocket guide for satellite cell research. In European journal of translational myology, 33, . doi:10.4081/ejtm.2023.12174. https://pubmed.ncbi.nlm.nih.gov/38112596/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen