C57BL/6JCya-Cdc27em1flox/Cya
Common Name:
Cdc27-flox
Product ID:
S-CKO-18782
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cdc27-flox
Strain ID
CKOCMP-217232-Cdc27-B6J-VB
Gene Name
Product ID
S-CKO-18782
Gene Alias
APC3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdc27em1flox/Cya mice (Catalog S-CKO-18782) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000093923
NCBI RefSeq
NM_145436
Target Region
Exon 6~9
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Cdc27, a core component of the Anaphase Promoting complex/cyclosome, plays a crucial role in controlling cell cycle transitions during cellular division [1]. This function is essential for normal cell growth and development, and its associated pathway is fundamental to cell cycle regulation. Genetic models, such as gene knockout in zebrafish, can be valuable for studying Cdc27.
In zebrafish, cdc27 knockout led to craniofacial malformation, spine deformity, and cardiac edema, phenotypes similar to Hemifacial microsomia (HFM) [4]. This indicates a functional link between Cdc27 and HFM, likely through inhibiting cranial neural crest cell (CNCC) proliferation and disrupting pharyngeal chondrocyte differentiation.
In various cancers, functional studies have also been revealing. For example, in colorectal cancer, knockdown of Cdc27 in HCT116 cells inhibited proliferation both in vitro and in vivo, arresting the G1/S phase transition via the accumulation of p21 [6]. In gastric cancer, silencing Cdc27 expression effectively inhibited cell proliferation, invasion, and metastasis in vitro and in vivo [5]. In T-cell lymphoblastic lymphoma, CDC27 promoted proliferation in vivo and in vitro, facilitated G1/S transition, and promoted the expression of Cyclin D1 and CDK4 [3]. In neuroblastoma, CDC27 promoted cell growth, metastasis, and sphere-formation ability in vitro and in vivo [2].
In conclusion, Cdc27 is essential for regulating cell cycle transitions. Studies using gene knockout models in zebrafish have revealed its role in HFM, while in vivo studies in cancer cell models have shown its significance in cancer progression. These findings highlight Cdc27's importance in both normal development and disease conditions, providing potential therapeutic targets for treating related diseases.
References:
1. Kazemi-Sefat, Golnaz Ensieh, Keramatipour, Mohammad, Talebi, Saeed, Kazemi-Sefat, Nazanin Atieh, Mousavizadeh, Kazem. 2021. The importance of CDC27 in cancer: molecular pathology and clinical aspects. In Cancer cell international, 21, 160. doi:10.1186/s12935-021-01860-9. https://pubmed.ncbi.nlm.nih.gov/33750395/
2. Qiu, Lin, Zhou, Rui, Luo, Ziyan, Wu, Jiangxue, Jiang, Hua. 2022. CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma. In Frontiers in oncology, 12, 774458. doi:10.3389/fonc.2022.774458. https://pubmed.ncbi.nlm.nih.gov/35242701/
3. Song, Yue, Song, Wei, Li, Zhaoming, Xia, Qingxin, Zhang, Mingzhi. 2020. CDC27 Promotes Tumor Progression and Affects PD-L1 Expression in T-Cell Lymphoblastic Lymphoma. In Frontiers in oncology, 10, 488. doi:10.3389/fonc.2020.00488. https://pubmed.ncbi.nlm.nih.gov/32391258/
4. Song, Wenjie, Xia, Xin, Fan, Yue, Zhang, Bo, Chen, Xiaowei. 2024. Functional and Genetic Analyses Unveil the Implication of CDC27 in Hemifacial Microsomia. In International journal of molecular sciences, 25, . doi:10.3390/ijms25094707. https://pubmed.ncbi.nlm.nih.gov/38731925/
5. Xin, Yongfan, Ning, Shili, Zhang, Liang, Cui, Ming. 2018. CDC27 Facilitates Gastric Cancer Cell Proliferation, Invasion and Metastasis via Twist-Induced Epithelial-Mesenchymal Transition. In Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 50, 501-511. doi:10.1159/000494164. https://pubmed.ncbi.nlm.nih.gov/30308498/
6. Qiu, L, Wu, J, Pan, C, Geng, R, Huang, W. 2016. Downregulation of CDC27 inhibits the proliferation of colorectal cancer cells via the accumulation of p21Cip1/Waf1. In Cell death & disease, 7, e2074. doi:10.1038/cddis.2015.402. https://pubmed.ncbi.nlm.nih.gov/26821069/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen