C57BL/6JCya-Rpl10em1flox/Cya
Common Name:
Rpl10-flox
Product ID:
S-CKO-18799
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rpl10-flox
Strain ID
CKOCMP-110954-Rpl10-B6J-VA
Gene Name
Product ID
S-CKO-18799
Gene Alias
D0HXS648; DXHXS648; DXHXS648E; QM; QM16
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rpl10em1flox/Cya mice (Catalog S-CKO-18799) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000008826
NCBI RefSeq
NM_052835
Target Region
Exon 1~2
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Rpl10, a ribosomal protein, is essential for ribosome function, playing a crucial role in protein translation. Ribosomes, where Rpl10 is located, are central to mRNA and protein quality control processes [2]. It is also involved in various signaling pathways, such as the JAK-STAT pathway in the context of T-cell acute lymphoblastic leukemia (T-ALL) [5].
In T-ALL, the R98S mutation in Rpl10 affects cellular proliferation, with cells accumulating reactive oxygen species, promoting mitochondrial dysfunction and reduced ATP levels [3]. These mutant leukemia cells survive high oxidative stress via increased IRES-mediated translation of BCL-2 [3]. Also, the R98S mutation enhances JAK-STAT signaling, as seen by overexpression of several Jak-Stat signaling proteins and hyper-activation of the pathway upon cytokine stimulation [5]. In pancreatic adenocarcinoma, the ufmylation of Rpl10 significantly increases cell proliferation and stemness, mainly due to higher expression of transcription factor KLF4 [1]. In plants like Nicotiana benthamiana and Arabidopsis, silencing or mutating Rpl10 compromises nonhost disease resistance, suggesting it positively regulates defence and translation-associated genes during nonhost pathogen infection [4].
In conclusion, Rpl10 is vital for ribosome-related functions and protein translation. Its study through genetic models, especially in diseases like T-ALL, pancreatic adenocarcinoma, and in understanding nonhost disease resistance in plants, has revealed its role in processes such as cell proliferation, stemness, and stress response. These findings contribute to a better understanding of disease mechanisms and may offer potential therapeutic targets.
References:
1. Wang, Kun, Chen, Siyu, Wu, Yue, Sun, Yanzi, Chen, Yijun. 2023. The ufmylation modification of ribosomal protein L10 in the development of pancreatic adenocarcinoma. In Cell death & disease, 14, 350. doi:10.1038/s41419-023-05877-y. https://pubmed.ncbi.nlm.nih.gov/37280198/
2. Yang, Yoon-Mo, Jung, Youngeun, Abegg, Daniel, Carroll, Kate S, Karbstein, Katrin. 2023. Chaperone-directed ribosome repair after oxidative damage. In Molecular cell, 83, 1527-1537.e5. doi:10.1016/j.molcel.2023.03.030. https://pubmed.ncbi.nlm.nih.gov/37086725/
3. Kampen, Kim R, Sulima, Sergey O, Verbelen, Benno, Vermeersch, Pieter, De Keersmaecker, Kim. 2018. The ribosomal RPL10 R98S mutation drives IRES-dependent BCL-2 translation in T-ALL. In Leukemia, 33, 319-332. doi:10.1038/s41375-018-0176-z. https://pubmed.ncbi.nlm.nih.gov/29930300/
4. Ramu, Vemanna S, Dawane, Akashata, Lee, Seonghee, Senthil-Kumar, Muthappa, Mysore, Kirankumar S. 2020. Ribosomal protein QM/RPL10 positively regulates defence and protein translation mechanisms during nonhost disease resistance. In Molecular plant pathology, 21, 1481-1494. doi:10.1111/mpp.12991. https://pubmed.ncbi.nlm.nih.gov/32964634/
5. Girardi, T, Vereecke, S, Sulima, S O, Kampen, K R, De Keersmaecker, K. 2017. The T-cell leukemia-associated ribosomal RPL10 R98S mutation enhances JAK-STAT signaling. In Leukemia, 32, 809-819. doi:10.1038/leu.2017.225. https://pubmed.ncbi.nlm.nih.gov/28744013/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen