C57BL/6JCya-Krt5em1flox/Cya
Common Name:
Krt5-flox
Product ID:
S-CKO-18809
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Krt5-flox
Strain ID
CKOCMP-110308-Krt5-B6J-VB
Gene Name
Product ID
S-CKO-18809
Gene Alias
3300001P10Rik; CK5; K5; Krt2-5; Tfip8
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Krt5em1flox/Cya mice (Catalog S-CKO-18809) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023709
NCBI RefSeq
NM_027011
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Krt5, also known as keratin 5, is a protein-coding gene. Keratins are intermediate filament proteins that play a crucial role in providing mechanical stability to epithelial cells. Krt5 is expressed in basal epithelial cells and is involved in maintaining the integrity of epithelial tissues. It may also be associated with signaling pathways, though specific pathways are context-dependent, and its overall biological importance lies in tissue development, homeostasis, and response to injury [2,3,4,5,6,7,8]. Genetic models, such as knockout (KO) and conditional knockout (CKO) mouse models, can be valuable in studying its function.
In lung-related studies, p63(+)Krt5(+) distal airway stem cells are essential for lung regeneration. Selective ablation of these cells in vivo prevents lung regeneration after influenza-induced damage, leading to pre-fibrotic lesions and deficient oxygen exchange, indicating the importance of Krt5-expressing cells in lung repair processes [1]. In melanoma, knockdown of Krt5 using siRNAs significantly promoted the proliferation, migration, and invasion of A375 cells, suggesting that Krt5 plays inhibitory roles in melanoma metastasis [4]. In the kidney, Krt5+ urothelial cells act as developmental and tissue repair progenitors. Fate mapping analysis showed that adult uroplakin-positive renal urothelial cells derive from embryonic and neonatal Krt5+ cells, and in response to congenital urinary tract obstruction, postnatal Krt5+ cells can differentiate into uroplakin-positive cells [8].
In conclusion, Krt5 is crucial for maintaining the integrity of epithelial tissues. Model-based research, especially KO/CKO mouse models, has revealed its significance in lung regeneration, melanoma metastasis, and kidney urothelial development and repair. Understanding Krt5's function provides insights into the mechanisms of these biological processes and related disease conditions.
References:
1. Zuo, Wei, Zhang, Ting, Wu, Daniel Zheng'An, Xian, Wa, McKeon, Frank. 2014. p63(+)Krt5(+) distal airway stem cells are essential for lung regeneration. In Nature, 517, 616-20. doi:10.1038/nature13903. https://pubmed.ncbi.nlm.nih.gov/25383540/
2. Jia, Weixue, Zhang, Yuanyuan, Wang, Xue, Mo, Ran, Li, Chengrang. 2023. KRT5 mutation regulate melanin metabolism through notch signalling pathway between keratinocytes and melanocytes. In Experimental dermatology, 32, 752-765. doi:10.1111/exd.14761. https://pubmed.ncbi.nlm.nih.gov/36809573/
3. Guo, Liuxiong, Liu, Yixuan, Yang, Tao, Liu, Bin, Cai, Jianhui. . CAV1 and KRT5 are potential targets for prostate cancer. In Medicine, 102, e36473. doi:10.1097/MD.0000000000036473. https://pubmed.ncbi.nlm.nih.gov/38065913/
4. Xie, Renjian, Li, Bifei, Jia, Lee, Li, Yumei. 2022. Identification of Core Genes and Pathways in Melanoma Metastasis via Bioinformatics Analysis. In International journal of molecular sciences, 23, . doi:10.3390/ijms23020794. https://pubmed.ncbi.nlm.nih.gov/35054979/
5. Moreno-Valladares, Manuel, Moncho-Amor, Veronica, Silva, Tulio M, Álvarez-Satta, María, Matheu, Ander. 2023. KRT5+/p63+ Stem Cells Undergo Senescence in the Human Lung with Pathological Aging. In Aging and disease, 14, 1013-1027. doi:10.14336/AD.2022.1128. https://pubmed.ncbi.nlm.nih.gov/37191411/
6. Salahudeen, Ameen A, Choi, Shannon S, Rustagi, Arjun, Desai, Tushar J, Kuo, Calvin J. 2020. Progenitor identification and SARS-CoV-2 infection in human distal lung organoids. In Nature, 588, 670-675. doi:10.1038/s41586-020-3014-1. https://pubmed.ncbi.nlm.nih.gov/33238290/
7. Kiener, Sarah, Mauldin, Elizabeth A, Jagannathan, Vidhya, Casal, Margret L, Leeb, Tosso. 2022. KRT5 missense variant in a Cardigan Welsh Corgi with epidermolysis bullosa simplex. In Animal genetics, 53, 892-896. doi:10.1111/age.13257. https://pubmed.ncbi.nlm.nih.gov/36004757/
8. Jackson, Ashley R, Hoff, Monica L, Li, Birong, McHugh, Kirk M, Becknell, Brian. 2019. Krt5+ urothelial cells are developmental and tissue repair progenitors in the kidney. In American journal of physiology. Renal physiology, 317, F757-F766. doi:10.1152/ajprenal.00171.2019. https://pubmed.ncbi.nlm.nih.gov/31322419/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen