C57BL/6JCya-Nhsl3em1flox/Cya
Common Name:
Nhsl3-flox
Product ID:
S-CKO-18812
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nhsl3-flox
Strain ID
CKOCMP-97130-Nhsl3-B6J-VA
Gene Name
Product ID
S-CKO-18812
Gene Alias
C77080; mKIAA1522
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nhsl3em1flox/Cya mice (Catalog S-CKO-18812) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000052602
NCBI RefSeq
NM_001033189
Target Region
Exon 5~7
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Nhsl3, a member of the Nance-Horan Syndrome family, is involved in controlling cell migration. Although the molecular mechanisms of cell migration are not fully understood, Nhsl3 seems to play a crucial role in this biological process [1].
Gene knockout experiments of Nhsl3 have revealed interesting findings. Nhsl3-knockout cells show increased persistence in single-cell migration compared to parental cells. However, in wound healing, follower cells lacking Nhsl3 are impaired in their ability to follow leader cells. The NHSL3 locus encodes several isoforms. Specific isoforms' interactions are critical for different types of cell migration. A long isoform's interaction with MENA/VASP proteins at cell-cell junctions is essential for collective migration, while a short isoform's interaction with 14-3-3θ in lamellipodia is crucial for single-cell migration [1].
In conclusion, Nhsl3 regulates single and collective cell migration through distinct mechanisms. The gene knockout models of Nhsl3 have provided valuable insights into the role of this gene in cell migration processes, which may potentially contribute to understanding diseases related to abnormal cell migration [1].
References：
1. Novikov, Nikita M, Gao, Jinmei, Fokin, Artem I, Guerois, Raphael, Gautreau, Alexis M. 2025. NHSL3 controls single and collective cell migration through two distinct mechanisms. In Nature communications, 16, 205. doi:10.1038/s41467-024-55647-3. https://pubmed.ncbi.nlm.nih.gov/39747206/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen