C57BL/6JCya-H6pdem1flox/Cya
Common Name:
H6pd-flox
Product ID:
S-CKO-18829
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
H6pd-flox
Strain ID
CKOCMP-100198-H6pd-B6J-VB
Gene Name
Product ID
S-CKO-18829
Gene Alias
G6pd1; Gpd-1; Gpd1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-H6pdem1flox/Cya mice (Catalog S-CKO-18829) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030830
NCBI RefSeq
NM_173371
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
H6PD, also known as hexose-6-phosphate dehydrogenase, is a microsomal enzyme that catalyzes the first two reactions of the oxidative chain of the pentose phosphate pathway [3]. This pathway is crucial for generating NADPH, which is involved in various biological processes such as antioxidant defense, steroid synthesis, and nucleotide biosynthesis.
In mice with a targeted mutation in H6PD, the oxo-reductase activity of 11β-hydroxysteroid dehydrogenase type 1 (11-HSD1) is drastically decreased, while its 11-dehydrogenase activity is increased. These mice share many phenotypic features with those having a mutation of 11-HSD1 itself, indicating H6PD's role in corticosteroid metabolism [2]. In human studies, a case-control study in Iranian Kurdish women found that variants of H6PD R453Q affect the risk of polycystic ovary syndrome (PCOS) [3]. Also, in gallbladder cancer, miR-551b-3p can target and inhibit H6PD expression, and overexpression of miR-551b-3p suppresses the epithelial-mesenchymal transition, migration, and invasion of gallbladder cancer cells [1].
In conclusion, H6PD is essential for the pentose phosphate pathway and thus for NADPH production. Its role in corticosteroid metabolism has been revealed through mouse models. Moreover, its gene polymorphisms are associated with PCOS, and its regulation by miRNAs is linked to gallbladder cancer progression. Understanding H6PD's functions provides insights into these disease mechanisms and may offer potential therapeutic targets.
References:
1. Ji, Tao, Gao, Lijun, Yu, Zongbu. 2020. Tumor-suppressive microRNA-551b-3p targets H6PD to inhibit gallbladder cancer progression. In Cancer gene therapy, 28, 693-705. doi:10.1038/s41417-020-00252-x. https://pubmed.ncbi.nlm.nih.gov/33250514/
2. White, Perrin C, Rogoff, Daniela, McMillan, D Randy, Lavery, Gareth G. 2007. Hexose 6-phosphate dehydrogenase (H6PD) and corticosteroid metabolism. In Molecular and cellular endocrinology, 265-266, 89-92. doi:. https://pubmed.ncbi.nlm.nih.gov/17240046/
3. Naseri, Rozita, Alimoradi, Yosra, Sohrabi, Maryam, Abdolmaleki, Amir, Jalili, Cyrus. 2022. H6PD Gene Polymorphisms (R453Q and D151A) and Polycystic Ovary Syndrome: A Case-Control Study in A Population of Iranian Kurdish Women. In International journal of fertility & sterility, 16, 180-183. doi:10.22074/ijfs.2021.141690.1050. https://pubmed.ncbi.nlm.nih.gov/36029054/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen