C57BL/6JCya-Atg7em1flox/Cya
Common Name:
Atg7-flox
Product ID:
S-CKO-18867
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Atg7-flox
Strain ID
CKOCMP-74244-Atg7-B6J-VA
Gene Name
Product ID
S-CKO-18867
Gene Alias
1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atg7em1flox/Cya mice (Catalog S-CKO-18867) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000169310
NCBI RefSeq
NM_001253717
Target Region
Exon 5~6
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Atg7, an autophagy-related gene, is essential for classical degradative macroautophagy and cell cycle regulation. It acts as an E1-like activating enzyme, facilitating the conjugation of microtubule-associated protein light chain 3 (LC3)-phosphatidylethanolamine and ATG12, standing at the hub of ubiquitin-like systems in autophagic vesicle expansion. Atg7 is involved in multiple biological processes such as autophagy, adipogenesis, angiogenesis, and immune responses, and is crucial for maintaining cellular homeostasis and organismal health [1,2,4,5,6].
In adipose-selective fto knockout mice, FTO deficiency decreased white fat mass and impaired ATG7-dependent autophagy, indicating that m6A methylation machinery regulates autophagy and adipogenesis through targeting Atg7 [1]. Endothelial cell-specific Atg7 knockout (atg7 KO) mice showed impaired angiogenesis, delayed blood flow reperfusion, and reduced HIF1A expression due to up-regulation of STAT1 [2]. In Atg7-ECKO mice, there was astrocyte-microvascular disassociation, BBB leakage, and down-regulation of fibronectin, suggesting Atg7 is required for maintaining BBB homeostasis [3].
In conclusion, Atg7 is central to autophagy and has diverse functions in multiple biological processes. Gene knockout models, especially KO and CKO mouse models, have revealed its roles in adipogenesis, angiogenesis, and blood-brain barrier maintenance. Understanding Atg7 provides insights into the mechanisms of related diseases, potentially leading to new therapeutic strategies for obesity, cancer, and cardiovascular diseases [1,2,3].
References:
1. Wang, Xinxia, Wu, Ruifan, Liu, Youhua, Wang, Fengqin, Wang, Yizhen. 2019. m6A mRNA methylation controls autophagy and adipogenesis by targeting Atg5 and Atg7. In Autophagy, 16, 1221-1235. doi:10.1080/15548627.2019.1659617. https://pubmed.ncbi.nlm.nih.gov/31451060/
2. Yao, Hongmin, Li, Jian, Liu, Zhixue, Mu, Jing, Xie, Zhonglin. 2022. Ablation of endothelial Atg7 inhibits ischemia-induced angiogenesis by upregulating Stat1 that suppresses Hif1a expression. In Autophagy, 19, 1491-1511. doi:10.1080/15548627.2022.2139920. https://pubmed.ncbi.nlm.nih.gov/36300763/
3. Liu, Hui, Wei, Jia-Yi, Li, Yuan, Zhao, Wei-Dong, Chen, Yu-Hua. 2023. Endothelial depletion of Atg7 triggers astrocyte-microvascular disassociation at blood-brain barrier. In The Journal of cell biology, 222, . doi:10.1083/jcb.202103098. https://pubmed.ncbi.nlm.nih.gov/36995368/
4. Chen, Jinxiang, Liang, Yu, Hu, Shaorun, Zeng, Min, Luo, Mao. 2024. Role of ATG7-dependent non-autophagic pathway in angiogenesis. In Frontiers in pharmacology, 14, 1266311. doi:10.3389/fphar.2023.1266311. https://pubmed.ncbi.nlm.nih.gov/38269279/
5. Collier, Jack J, Suomi, Fumi, Oláhová, Monika, McWilliams, Thomas G, Taylor, Robert W. 2021. Emerging roles of ATG7 in human health and disease. In EMBO molecular medicine, 13, e14824. doi:10.15252/emmm.202114824. https://pubmed.ncbi.nlm.nih.gov/34725936/
6. Xiong, Jianhua. 2015. Atg7 in development and disease: panacea or Pandora's Box? In Protein & cell, 6, 722-34. doi:10.1007/s13238-015-0195-8. https://pubmed.ncbi.nlm.nih.gov/26404030/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen