C57BL/6JCya-Ino80dem1flox/Cya
Common Name:
Ino80d-flox
Product ID:
S-CKO-18873
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ino80d-flox
Strain ID
CKOCMP-227195-Ino80d-B6J-VB
Gene Name
Product ID
S-CKO-18873
Gene Alias
7330405I11; A430093A21Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ino80dem1flox/Cya mice (Catalog S-CKO-18873) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000097718
NCBI RefSeq
NM_001406704
Target Region
Exon 5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Ino80d, a component of the INO80 chromatin remodeling complex, is crucial for chromatin remodeling, gene expression, and DNA repair. It is involved in processes such as spermatogenesis, and its encoded protein is related to DNA binding, chromatin modification, chromosome structure organization, and ATP-dependent nucleosome sliding [1,3].
In Chinese mitten crab spermatogenesis, INO80D is initially involved in chromatin decondensation for gene accessibility and DNA repair in early spermatogenesis, and later maintains decondensed chromatin stability during spermiogenesis [1].
In mice, its expression is up-regulated in adult testis tissue, and miR-92a-3p may negatively regulate it, influencing testicular development and spermatogenesis [2].
A missense mutation (Ser818Cys) in INO80D in humans is linked to a syndrome of aortic hypoplasia, premature atherosclerosis, and arterial stiffness, suggesting its role in arterial aging [3].
In translocation renal cell carcinoma, mutations in INO80D were identified, and knockdown of INO80D decreased cell proliferation in a relevant cell line [4].
In summary, Ino80d plays multifaceted roles in spermatogenesis and is associated with certain human diseases. Studies on Ino80d in various species contribute to understanding its functions in biological processes and disease mechanisms, potentially providing insights for related disease treatment and prevention [1,2,3,4].
References：
1. Mo, Yinyin, Sun, Lishuang, Li, Shu, Chen, Zhengyu, Li, Genliang. 2024. The mechanism of INO80D involved in chromatin remodeling regulating spermatogenesis in Chinese mitten crab (Eriocheir sinensis). In Molecular genetics and genomics : MGG, 299, 83. doi:10.1007/s00438-024-02177-8. https://pubmed.ncbi.nlm.nih.gov/39212752/
2. Luo, Lvjing, Sun, Lishuang, Li, Shu, Mo, Yinyin, Li, Genliang. 2024. Localization and functional analysis of miR-92a-3p regulating Ino80d in mouse testis. In Reproductive biology, 24, 100961. doi:10.1016/j.repbio.2024.100961. https://pubmed.ncbi.nlm.nih.gov/39405922/
3. Shameer, Khader, Klee, Eric W, Dalenberg, Angela K, Kullo, Iftikhar J. 2014. Whole exome sequencing implicates an INO80D mutation in a syndrome of aortic hypoplasia, premature atherosclerosis, and arterial stiffness. In Circulation. Cardiovascular genetics, 7, 607-14. doi:10.1161/CIRCGENETICS.113.000233. https://pubmed.ncbi.nlm.nih.gov/25122053/
4. Malouf, Gabriel G, Su, Xiaoping, Yao, Hui, Weinstein, John, Tannir, Nizar M. 2014. Next-generation sequencing of translocation renal cell carcinoma reveals novel RNA splicing partners and frequent mutations of chromatin-remodeling genes. In Clinical cancer research : an official journal of the American Association for Cancer Research, 20, 4129-40. doi:10.1158/1078-0432.CCR-13-3036. https://pubmed.ncbi.nlm.nih.gov/24899691/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen