C57BL/6JCya-Slc34a2em1flox/Cya
Common Name:
Slc34a2-flox
Product ID:
S-CKO-18880
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc34a2-flox
Strain ID
CKOCMP-20531-Slc34a2-B6J-VB
Gene Name
Product ID
S-CKO-18880
Gene Alias
D5Ertd227e; NaPi-2b; Npt2b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc34a2em1flox/Cya mice (Catalog S-CKO-18880) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000094787
NCBI RefSeq
NM_011402
Target Region
Exon 5~6
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Slc34a2, encoding the sodium-dependent phosphate transport protein 2B (NaPi-2b), is a gene involved in the sodium-driven phosphate cotransport process. It plays a crucial role in maintaining phosphate homeostasis, especially in intestinal phosphate absorption [1]. In the context of disease, it has been associated with pathways related to cancer cell proliferation, cell cycle progression, and autophagy, as well as a rare autosomal recessive lung disease, pulmonary alveolar microlithiasis (PAM) [2-10]. Genetic models, such as knockout (KO) or conditional knockout (CKO) mouse models, are valuable for studying its functions.
A conditional NaPi-IIb (encoded by Slc34a2) knockout mouse demonstrated that this protein is vital for maintaining skeletal integrity during phosphate restriction. Under normal physiological conditions, the passive sodium-independent pathway may be more dominant for intestinal phosphate absorption [1]. In PAM, mutations in Slc34a2 are considered responsible. For example, a homozygous mutation c.910A > T (p.K304X) was identified in Chinese PAM patients [2]. In cancer research, loss-of-function experiments in various cancer cell lines like colorectal, esophageal, and papillary thyroid carcinoma cells have shown that Slc34a2 promotes cancer cell proliferation, cell cycle progression, and invasion through different mechanisms. For instance, in colorectal cancer, it targets TMPRSS3, and in esophageal squamous cell carcinoma, it activates STX17-mediated autophagy [3,4].
In conclusion, Slc34a2 is essential for phosphate homeostasis, especially in intestinal phosphate absorption and skeletal integrity maintenance. Its mutations are closely related to PAM, and its abnormal expression promotes cancer development in multiple types of cancers. The study of Slc34a2 using KO/CKO mouse models has significantly contributed to understanding its roles in these biological processes and disease conditions.
References:
1. Marks, Joanne. 2018. The role of SLC34A2 in intestinal phosphate absorption and phosphate homeostasis. In Pflugers Archiv : European journal of physiology, 471, 165-173. doi:10.1007/s00424-018-2221-1. https://pubmed.ncbi.nlm.nih.gov/30343332/
2. Yin, Xinzhen, Wang, Huiying, Wu, Dingwen, Shao, Jingxin, Dai, Yu. 2012. SLC34A2 Gene mutation of pulmonary alveolar microlithiasis: report of four cases and review of literatures. In Respiratory medicine, 107, 217-22. doi:10.1016/j.rmed.2012.10.016. https://pubmed.ncbi.nlm.nih.gov/23164546/
3. Yang, Yi, Wu, Jiang, Yu, Xiaofeng, Dai, Xinyi, Chen, Haijun. 2021. SLC34A2 promotes cancer proliferation and cell cycle progression by targeting TMPRSS3 in colorectal cancer. In Pathology, research and practice, 229, 153706. doi:10.1016/j.prp.2021.153706. https://pubmed.ncbi.nlm.nih.gov/34929599/
4. Xu, Yi, Duan, Shiyu, Ye, Wen, Gao, Ying, Ye, Sheng. 2024. SLC34A2 promotes cell proliferation by activating STX17-mediated autophagy in esophageal squamous cell carcinoma. In Thoracic cancer, 15, 1369-1384. doi:10.1111/1759-7714.15314. https://pubmed.ncbi.nlm.nih.gov/38720472/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen