C57BL/6JCya-Srsf2em1flox/Cya
Common Name:
Srsf2-flox
Product ID:
S-CKO-18893
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Srsf2-flox
Strain ID
CKOCMP-20382-Srsf2-B6J-VA
Gene Name
Product ID
S-CKO-18893
Gene Alias
D11Wsu175e; MRF-1; Pr264; SC35; Sfrs10; Sfrs2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Srsf2em1flox/Cya mice (Catalog S-CKO-18893) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000092404
NCBI RefSeq
NM_011358
Target Region
Exon 1~2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Srsf2, also known as SC35, is a serine/arginine-rich splicing factor widely expressed in mammalian cells. It plays vital roles in pre-mRNA processing and gene transcription, participating in numerous biological processes such as spermatogenesis, cell-cycle regulation, and DNA damage response [3,4]. It has also been implicated in disease-related pathways, especially in myelodysplastic syndromes (MDS) and myeloproliferative neoplasms (MPN) [1,2]. Genetic models, like KO/CKO mouse models, are valuable for studying its functions.
In male germ cell-specific Srsf2 knockout (Srsf2-deleted by Stra8-Cre), mice showed complete infertility and defective spermatogenesis. Srsf2 regulatory networks were found crucial for reproductive development, spermatogenesis, and related events. Srsf2 directly affected the expression and alternative splicing of genes encoding critical spermatogenesis factors [3].
In Srsf2P95H mutant knock-in mice co-expressed with Jak2V617F, reduced red blood cell, neutrophil, and platelet counts were observed, along with attenuated splenomegaly, without bone marrow fibrosis induction. Srsf2P95H also diminished the competitiveness of Jak2V617F mutant hematopoietic stem/progenitor cells [5].
Srsf2P95H in another set of knock-in mice delayed myelofibrosis induced by Jak2V617F, decreased TGFβ1 serum level, and reduced the competitiveness of transplanted Jak2V617F hematopoietic stem cells while preventing their exhaustion. RNA sequencing of megakaryocytes showed an increased number of splicing events when the two mutations were combined, with Srsf2P95H promoting Jak2 exon 14 skipping, generating an inactive JAK2 protein [6].
In conclusion, Srsf2 is essential for mRNA splicing during spermatogenesis and has a significant impact on hematopoietic functions as revealed through mouse model-based research. In disease areas, especially MDS and MPN, Srsf2 mutations play important roles, and studies using KO/CKO mouse models have provided insights into the molecular mechanisms underlying these diseases, which may help develop new therapeutic strategies.
References:
1. Kim, Eunhee, Ilagan, Janine O, Liang, Yang, Bradley, Robert K, Abdel-Wahab, Omar. . SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific Effects on Exon Recognition. In Cancer cell, 27, 617-30. doi:10.1016/j.ccell.2015.04.006. https://pubmed.ncbi.nlm.nih.gov/25965569/
2. Aujla, Amandeep, Linder, Katherine, Iragavarapu, Chaitanya, Karass, Michael, Liu, Delong. 2018. SRSF2 mutations in myelodysplasia/myeloproliferative neoplasms. In Biomarker research, 6, 29. doi:10.1186/s40364-018-0142-y. https://pubmed.ncbi.nlm.nih.gov/30275952/
3. Lei, Wen-Long, Du, Zongchang, Meng, Tie-Gang, Qian, Wei-Ping, Sun, Qing-Yuan. 2023. SRSF2 is required for mRNA splicing during spermatogenesis. In BMC biology, 21, 231. doi:10.1186/s12915-023-01736-6. https://pubmed.ncbi.nlm.nih.gov/37867192/
4. Wagner, Rebecca E, Arnetzl, Leonie, Britto-Borges, Thiago, Bornelöv, Susanne, Frye, Michaela. 2024. SRSF2 safeguards efficient transcription of DNA damage and repair genes. In Cell reports, 43, 114869. doi:10.1016/j.celrep.2024.114869. https://pubmed.ncbi.nlm.nih.gov/39446588/
5. Yang, Yue, Abbas, Salar, Sayem, Mohammad A, Dutta, Avik, Mohi, Golam. 2023. SRSF2 mutation reduces polycythemia and impairs hematopoietic progenitor functions in JAK2V617F-driven myeloproliferative neoplasm. In Blood cancer journal, 13, 171. doi:10.1038/s41408-023-00947-y. https://pubmed.ncbi.nlm.nih.gov/38012156/
6. Willekens, Christophe, Laplane, Lucie, Dagher, Tracy, Vainchenker, William, Solary, Eric. 2023. SRSF2-P95H decreases JAK/STAT signaling in hematopoietic cells and delays myelofibrosis development in mice. In Leukemia, 37, 1287-1297. doi:10.1038/s41375-023-01878-0. https://pubmed.ncbi.nlm.nih.gov/37100881/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen