C57BL/6JCya-Elp3em1flox/Cya
Common Name:
Elp3-flox
Product ID:
S-CKO-18920
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Elp3-flox
Strain ID
CKOCMP-74195-Elp3-B6J-VB
Gene Name
Product ID
S-CKO-18920
Gene Alias
2610507P14Rik; KAT9
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Elp3em1flox/Cya mice (Catalog S-CKO-18920) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022609
NCBI RefSeq
NM_001253812
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Elp3, also known as Elongator protein 3 or Kat9, is the catalytic subunit of the Elongator complex. It functions as an acetyltransferase and a tRNA-modifying enzyme, modifying the wobble uridine base U34 in cytosolic tRNAs. Elp3 is involved in multiple pathways such as mTORC2 activation, Wnt signaling, and is important for processes like macrophage polarization, cell metabolism, and tumorigenesis. Genetic models, especially KO/CKO mouse models, have been crucial in understanding its functions [1-6, 8].
In myeloid cells of mice, Elp3 expression is downregulated by M1-activating signals, limiting pro-inflammatory cytokines production via FoxO1 phosphorylation and attenuating experimental colitis. In contrast, M2-activating signals upregulate its expression, and M2 macrophage polarization-linked metabolic reprogramming depends on Elp3. Elp3 also promotes mTORC2 activation by codon-dependent translation of its activator Ric8b and further promotes Wnt-driven tumor initiation in the intestine [1]. In another study, Elp3 knockdown in mice diminished glycolysis and glutaminolysis, retarded cell proliferation and xenograft growth by downregulating c-Myc, as it stabilizes c-Myc in an acetyltransferase-independent manner [2]. Loss of epithelial Elp3 in mouse intestine blocks intestinal tuft cell differentiation via an mTORC1-Atf4 axis, as it is essential for immature tuft cell generation and IL-13-dependent induction of glycolytic enzymes [3]. In hematopoietic system of mice, loss of Elp3 causes bone marrow failure, activates a p53-dependent checkpoint, and can lead to development of p53-mutated leukemia/lymphoma [4].
In conclusion, Elp3 plays essential roles in macrophage polarization, cell metabolism, tumorigenesis, and hematopoiesis. Mouse models with Elp3 KO/CKO have provided valuable insights into its functions in these processes and associated disease conditions such as colitis, cancer, and bone marrow failure, helping to understand the underlying mechanisms and potentially identify new therapeutic targets.
References:
1. Chen, Dawei, Nemazanyy, Ivan, Peulen, Olivier, Dewals, Benjamin, Chariot, Alain. 2022. Elp3-mediated codon-dependent translation promotes mTORC2 activation and regulates macrophage polarization. In The EMBO journal, 41, e109353. doi:10.15252/embj.2021109353. https://pubmed.ncbi.nlm.nih.gov/35920020/
2. Zhao, Wentao, Ouyang, Cong, Huang, Chen, Jiang, Bin, Li, Qinxi. . ELP3 stabilizes c-Myc to promote tumorigenesis. In Journal of molecular cell biology, 15, . doi:10.1093/jmcb/mjad059. https://pubmed.ncbi.nlm.nih.gov/37771073/
3. Wathieu, Caroline, Lavergne, Arnaud, Xu, Xinyi, Dewals, Benjamin G, Chariot, Alain. 2024. Loss of Elp3 blocks intestinal tuft cell differentiation via an mTORC1-Atf4 axis. In The EMBO journal, 43, 3916-3947. doi:10.1038/s44318-024-00184-4. https://pubmed.ncbi.nlm.nih.gov/39085648/
4. Rosu, Adeline, El Hachem, Najla, Rapino, Francesca, Bureau, Fabrice, Desmet, Christophe J. . Loss of tRNA-modifying enzyme Elp3 activates a p53-dependent antitumor checkpoint in hematopoiesis. In The Journal of experimental medicine, 218, . doi:10.1084/jem.20200662. https://pubmed.ncbi.nlm.nih.gov/33507234/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen