C57BL/6JCya-Wdfy4em1flox/Cya
Common Name:
Wdfy4-flox
Product ID:
S-CKO-18938
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Wdfy4-flox
Strain ID
CKOCMP-545030-Wdfy4-B6J-VB
Gene Name
Product ID
S-CKO-18938
Gene Alias
Gm18810
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Wdfy4em1flox/Cya mice (Catalog S-CKO-18938) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000061753
NCBI RefSeq
NM_001146022
Target Region
Exon 5~6
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
WDFY4, also known as WD repeat- and FYVE domain-containing protein 4, is a member of the BEACH (Beige and Chediak-Higashi) domain-containing family. It plays essential roles in the immune system, especially in the cross-presentation of classic dendritic cells, reactive oxygen species-induced apoptosis of CD8+ T cells, and non-canonical autophagic activity in B cells [3].
In gene knockout (KO) mouse models, Wdfy4 -/- mice fail to prime virus-specific CD8+ T cells in vivo or induce tumor rejection, indicating its crucial role in cross-presentation for antiviral and antitumor immunity [1]. In NOD mice, inactivation of Wdfy4 using Nuclease technology (NOD.Wdfy4 -/-) ameliorates autoimmune diabetes and insulitis, suggesting that priming of autoreactive CD8+ T cells in NOD mice requires cross-presentation by cDC1, which depends on Wdfy4 [2]. Lack of Wdfy4 in mice promotes Th2 cell differentiation, aggravating ovalbumin-induced asthma [4]. Also, selective deficiency of Wdfy4 in T cells leads to a reduction in the number of CD8+ T cells via reactive oxygen species-induced apoptosis, promoting tumor growth [5]. In B cells, loss of Wdfy4 in Wdfy4 conditional knockout (CKO) mice decreases the number of total B cells and some B cell subpopulations, alleviating SLE phenotypes [6].
In conclusion, WDFY4 is vital for multiple immune-related functions, including cross-presentation, T-cell and B-cell regulation. The use of Wdfy4 KO and CKO mouse models has significantly advanced our understanding of its roles in antiviral, antitumor immunity, and autoimmune diseases such as diabetes, asthma, and SLE.
References:
1. Theisen, Derek J, Davidson, Jesse T, Briseño, Carlos G, Murphy, Theresa L, Murphy, Kenneth M. . WDFY4 is required for cross-presentation in response to viral and tumor antigens. In Science (New York, N.Y.), 362, 694-699. doi:10.1126/science.aat5030. https://pubmed.ncbi.nlm.nih.gov/30409884/
2. Ferris, Stephen T, Liu, Tiantian, Chen, Jing, Murphy, Theresa L, Murphy, Kenneth M. 2023. WDFY4 deficiency in NOD mice ameliorates autoimmune diabetes and insulitis. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2219956120. doi:10.1073/pnas.2219956120. https://pubmed.ncbi.nlm.nih.gov/36940342/
3. Lyu, Xia, Lamb, Janine A, Chinoy, Hector. . The clinical relevance of WDFY4 in autoimmune diseases in diverse ancestral populations. In Rheumatology (Oxford, England), 63, 3255-3262. doi:10.1093/rheumatology/keae183. https://pubmed.ncbi.nlm.nih.gov/38507703/
4. Li, Yan, Wang, Anran, Long, Feng, Li, Jiangxia, Liu, Qiji. 2021. Lack of WDFY4 Aggravates Ovalbumin-Induced Asthma via Enhanced Th2 Cell Differentiation. In International archives of allergy and immunology, 182, 1089-1096. doi:10.1159/000516970. https://pubmed.ncbi.nlm.nih.gov/34425575/
5. Li, Yan, Li, Jiangxia, Yuan, Qianqian, Sun, Wenjie, Liu, Qiji. 2021. Deficiency in WDFY4 reduces the number of CD8+ T cells via reactive oxygen species-induced apoptosis. In Molecular immunology, 139, 131-138. doi:10.1016/j.molimm.2021.08.022. https://pubmed.ncbi.nlm.nih.gov/34482201/
6. Yuan, Qianqian, Li, Yan, Li, Jiangxia, Sun, Wenjie, Liu, Qiji. 2018. WDFY4 Is Involved in Symptoms of Systemic Lupus Erythematosus by Modulating B Cell Fate via Noncanonical Autophagy. In Journal of immunology (Baltimore, Md. : 1950), 201, 2570-2578. doi:10.4049/jimmunol.1800399. https://pubmed.ncbi.nlm.nih.gov/30257884/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen