C57BL/6JCya-Ttkem1flox/Cya
Common Name:
Ttk-flox
Product ID:
S-CKO-18972
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Ttk-flox
Strain ID
CKOCMP-22137-Ttk-B6J-VB
Gene Name
Product ID
S-CKO-18972
Gene Alias
Esk1; Mps1; PYT; esk
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ttkem1flox/Cya mice (Catalog S-CKO-18972) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070326
NCBI RefSeq
NM_009445
Target Region
Exon 5~8
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
TTK, also known as monopolar spindle 1 (hMps1), is a serine/threonine kinase. It is an integral part of the spindle assembly checkpoint signaling pathway, playing a crucial role in cell cycle regulation. Abnormal TTK expression has been associated with various biological processes and diseases [1,2,3,4,5,6,7,8].
In endometrial cancer, high TTK expression is linked to lower patient survival, and it can potentially serve as a biomarker for early detection. Repressing TTK expression in EC cells reduces cell proliferation, invasion, EMT, and chemoresistance [1].
In pancreatic ductal adenocarcinoma, H3K18la activates the transcription of TTK, and TTK forms a positive feedback loop with glycolysis, exacerbating oncogenesis [2].
In colorectal cancer, targeted depletion of TTK impairs cell proliferation, cell cycle progression, and organoid formation [3].
In liver cancer, TTK inhibitor sensitizes cells to radiation through regulating cell cycle in a p21-mediated manner [4].
In esophageal cancer, the ANXA2/TTK complex activates the Akt/mTOR signaling pathway, promoting cancer progression [5].
In glioblastoma, TTK promotes temozolomide resistance by inducing autophagy [6].
In vascular smooth muscle cells, TTK upregulation phosphorylates p120-catenin, and TTK inhibitor mitigates neointimal formation and atherosclerotic lesions [7].
In cisplatin-resistant ovarian cancer cells, down-regulation of TTK can recover cisplatin sensitivity [8].
In conclusion, TTK is a key regulator in multiple biological processes, especially in cell cycle-related events. Through gene-knockout or other loss-of-function experiments in various disease models, it has been shown to play significant roles in cancers such as endometrial, pancreatic, colorectal, liver, esophageal, glioblastoma, and ovarian cancer, as well as in atherosclerosis. Understanding TTK's function provides potential therapeutic targets for these diseases.
References:
1. Miao, Yu, Konno, Yosuke, Wang, Baojin, Dong, Peixin, Fang, Mingyan. 2023. Integrated multi-omics analyses and functional validation reveal TTK as a novel EMT activator for endometrial cancer. In Journal of translational medicine, 21, 151. doi:10.1186/s12967-023-03998-8. https://pubmed.ncbi.nlm.nih.gov/36829176/
2. Li, Fei, Si, Wenzhe, Xia, Li, Hong, Tianpei, Wei, Rui. 2024. Positive feedback regulation between glycolysis and histone lactylation drives oncogenesis in pancreatic ductal adenocarcinoma. In Molecular cancer, 23, 90. doi:10.1186/s12943-024-02008-9. https://pubmed.ncbi.nlm.nih.gov/38711083/
3. Shaath, Hibah, Vishnubalaji, Radhakrishnan, Elango, Ramesh, Jithesh, Puthen Veettil, Alajez, Nehad M. 2023. Therapeutic targeting of the TPX2/TTK network in colorectal cancer. In Cell communication and signaling : CCS, 21, 265. doi:10.1186/s12964-023-01290-2. https://pubmed.ncbi.nlm.nih.gov/37770979/
4. Zhang, Haibo, Yao, Weiping, Zhang, Minjun, You, Guangxian, Liang, Xiaodong. 2021. TTK inhibitor promotes radiosensitivity of liver cancer cells through p21. In Biochemical and biophysical research communications, 550, 84-91. doi:10.1016/j.bbrc.2021.01.089. https://pubmed.ncbi.nlm.nih.gov/33689884/
5. Liu, Ruiqi, Lu, Yanwei, Li, Jing, Liang, Xiaodong, Zhang, Haibo. 2024. Annexin A2 combined with TTK accelerates esophageal cancer progression via the Akt/mTOR signaling pathway. In Cell death & disease, 15, 291. doi:10.1038/s41419-024-06683-w. https://pubmed.ncbi.nlm.nih.gov/38658569/
6. Yu, Jian, Gao, Ge, Wei, Xiangpin, Wang, Yang. 2022. TTK Protein Kinase promotes temozolomide resistance through inducing autophagy in glioblastoma. In BMC cancer, 22, 786. doi:10.1186/s12885-022-09899-1. https://pubmed.ncbi.nlm.nih.gov/35850753/
7. Wu, Jie-Hong, Liu, Yu-Xiao, Zong, Jia-Bin, Zhao, Xin-Ran, Hu, Bo. 2024. TTK Inhibition Alleviates Postinjury Neointimal Formation and Atherosclerosis. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 12, e2409250. doi:10.1002/advs.202409250. https://pubmed.ncbi.nlm.nih.gov/39716891/
8. Liu, Yixuan, Zhu, Keyu, Guan, Xiaolin, Zheng, Hui, Lu, Renquan. 2021. TTK is a potential therapeutic target for cisplatin-resistant ovarian cancer. In Journal of ovarian research, 14, 128. doi:10.1186/s13048-021-00884-z. https://pubmed.ncbi.nlm.nih.gov/34598710/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen