C57BL/6JCya-Fitm1em1flox/Cya
Common Name:
Fitm1-flox
Product ID:
S-CKO-18974
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fitm1-flox
Strain ID
CKOCMP-68680-Fitm1-B6J-VA
Gene Name
Product ID
S-CKO-18974
Gene Alias
1110028A07Rik; Cg10671; Fit1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fitm1em1flox/Cya mice (Catalog S-CKO-18974) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022826
NCBI RefSeq
NM_026808
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Fitm1, also known as fat storage-inducing transmembrane protein 1, is a transmembrane endoplasmic/sarcoplasmic reticulum protein involved in lipid droplet formation [3,4,5]. It belongs to a family of proteins that play important roles in lipid metabolism. Lipid droplets are dynamic organelles whose formation and growth are regulated processes, and Fitm1 is one of the proteins that may serve as a potential therapeutic target for diseases related to abnormal lipid metabolism, such as non-alcoholic fatty liver disease (NAFLD) [3].
In non-viral hepatocellular carcinoma (HCC), Fitm1 was identified as a novel tumor suppressor gene. Through a series of analyses including PCA, Uni-variate, Multi-variate, and LASSO cox regression, a FITM1-methylation-based signature was built and validated, which can predict the prognosis of non-viral HCC patients [1]. In pancreatic cancer, Fitm1 was among the down-regulated genes in cancer tissues compared to normal pancreatic tissues. Bioinformatics analysis showed that its down-regulation was significantly associated with the overall survival of pancreatic cancer patients [2]. In mice, neither constitutive Fitm1 (-/-) aged nor heart failure model mice showed significant differences in heart size or function, indicating a minor role of Fitm1 in heart-related chronic heart failure compared to Fitm2 [4].
In conclusion, Fitm1 is mainly involved in lipid droplet formation and lipid metabolism. Its role as a tumor suppressor in non-viral HCC and its association with the prognosis of pancreatic cancer patients, as well as its minor impact on heart function in mice, have been revealed through various research models. These findings contribute to understanding the biological functions of Fitm1 in different disease conditions and may provide new perspectives for disease treatment and prognosis prediction.
References:
1. Chen, Jie, Wang, Xicheng, Wang, Xining, Chen, Tao, Chen, Yajin. 2020. A FITM1-Related Methylation Signature Predicts the Prognosis of Patients With Non-Viral Hepatocellular Carcinoma. In Frontiers in genetics, 11, 99. doi:10.3389/fgene.2020.00099. https://pubmed.ncbi.nlm.nih.gov/32174969/
2. Bai, Rubing, Rebelo, Artur, Kleeff, Jörg, Sunami, Yoshiaki. 2021. Identification of prognostic lipid droplet-associated genes in pancreatic cancer patients via bioinformatics analysis. In Lipids in health and disease, 20, 58. doi:10.1186/s12944-021-01476-y. https://pubmed.ncbi.nlm.nih.gov/34078402/
3. Goh, Vera J, Silver, David L. 2013. The lipid droplet as a potential therapeutic target in NAFLD. In Seminars in liver disease, 33, 312-20. doi:10.1055/s-0033-1358521. https://pubmed.ncbi.nlm.nih.gov/24222089/
4. Nishihama, Natsumi, Nagayama, Takahiro, Makino, Shinji, Koishi, Ryuta. 2019. Mice lacking fat storage-inducing transmembrane protein 2 show improved profiles upon pressure overload-induced heart failure. In Heliyon, 5, e01292. doi:10.1016/j.heliyon.2019.e01292. https://pubmed.ncbi.nlm.nih.gov/30923760/
5. Gross, David A, Snapp, Erik L, Silver, David L. 2010. Structural insights into triglyceride storage mediated by fat storage-inducing transmembrane (FIT) protein 2. In PloS one, 5, e10796. doi:10.1371/journal.pone.0010796. https://pubmed.ncbi.nlm.nih.gov/20520733/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen