C57BL/6JCya-Dpydem1flox/Cya
Common Name:
Dpyd-flox
Product ID:
S-CKO-18980
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dpyd-flox
Strain ID
CKOCMP-99586-Dpyd-B6J-VB
Gene Name
Product ID
S-CKO-18980
Gene Alias
DHPDHase; DPD; E330028L06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dpydem1flox/Cya mice (Catalog S-CKO-18980) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000039177
NCBI RefSeq
NM_170778
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
DPYD, which encodes dihydropyrimidine dehydrogenase (DPD), is a crucial gene. DPD is the rate-limiting enzyme for the metabolism of fluoropyrimidines like 5-fluorouracil and capecitabine. Its function in this metabolic pathway is essential for the proper breakdown of these anticancer drugs, thus influencing their efficacy and toxicity in cancer treatment [4,5].
Fluoropyrimidine treatment can lead to severe toxicity in up to 30% of patients, often due to reduced DPD activity caused by genetic variants in DPYD. Prospective screening for four relevant DPYD variants (DPYD*2A [rs3918290, c.1905 + 1G>A, IVS14 + 1G>A], c.2846A>T [rs67376798, D949V], c.1679T>G [rs55886062, DPYD*13, I560S], and c.1236G>A [rs56038477, E412E, in haplotype B3]) in Dutch cancer patients showed that DPYD genotype-guided dose individualisation improved patient safety. DPYD variant carriers had a higher frequency of severe fluoropyrimidine-related toxicity compared to wild-type patients. For DPYD*2A and c.1679T>G carriers, a 50% initial dose reduction was adequate, while for c.1236G>A and c.2846A>T carriers, a larger dose reduction might be needed [1]. In a matched-pair analysis, DPYD-guided fluoropyrimidine dosing did not negatively affect progression-free survival (PFS) and overall survival (OS) in pooled DPYD variant carriers, though c.1236G>A carriers had a shorter PFS [2]. A study in Canadian patients also found that a DPYD exon 4 deletion was associated with fluoropyrimidine toxicity, suggesting that copy number variation in DPYD may be an underappreciated determinant of toxicity [3].
In conclusion, DPYD is essential for the metabolism of fluoropyrimidines. Research on DPYD-related genetic variants through patient-based studies has shown its significance in guiding fluoropyrimidine-based cancer treatment. Understanding DPYD gene function and its variants helps in improving the safety and efficacy of fluoropyrimidine therapy for cancer patients.
References:
1. Henricks, Linda M, Lunenburg, Carin A T C, de Man, Femke M, Guchelaar, Henk-Jan, Schellens, Jan H M. 2018. DPYD genotype-guided dose individualisation of fluoropyrimidine therapy in patients with cancer: a prospective safety analysis. In The Lancet. Oncology, 19, 1459-1467. doi:10.1016/S1470-2045(18)30686-7. https://pubmed.ncbi.nlm.nih.gov/30348537/
2. Knikman, Jonathan E, Wilting, Tycho A, Lopez-Yurda, Marta, Guchelaar, Henk-Jan, Cats, Annemieke. 2023. Survival of Patients With Cancer With DPYD Variant Alleles and Dose-Individualized Fluoropyrimidine Therapy-A Matched-Pair Analysis. In Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 41, 5411-5421. doi:10.1200/JCO.22.02780. https://pubmed.ncbi.nlm.nih.gov/37639651/
3. Wigle, Theodore J, Medwid, Samantha, Ross, Cameron, Schwarz, Ute I, Kim, Richard B. 2023. DPYD Exon 4 Deletion Associated with Fluoropyrimidine Toxicity and Importance of Copy Number Variation. In Current oncology (Toronto, Ont.), 30, 663-672. doi:10.3390/curroncol30010051. https://pubmed.ncbi.nlm.nih.gov/36661700/
4. Lešnjaković, Lucija, Ganoci, Lana, Bilić, Ivan, Pleština, Stjepko, Božina, Nada. 2023. DPYD genotyping and predicting fluoropyrimidine toxicity: where do we stand? In Pharmacogenomics, 24, 93-106. doi:10.2217/pgs-2022-0135. https://pubmed.ncbi.nlm.nih.gov/36636997/
5. Turner, Amy J, Haidar, Cyrine E, Yang, Wenjian, Broeckel, Ulrich, Gaedigk, Andrea. . Updated DPYD HapB3 haplotype structure and implications for pharmacogenomic testing. In Clinical and translational science, 17, e13699. doi:10.1111/cts.13699. https://pubmed.ncbi.nlm.nih.gov/38129972/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen