C57BL/6JCya-Rnf149em1flox/Cya
Common Name:
Rnf149-flox
Product ID:
S-CKO-19004
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rnf149-flox
Strain ID
CKOCMP-67702-Rnf149-B6J-VB
Gene Name
Product ID
S-CKO-19004
Gene Alias
1600023E10Rik; Gm15832; Greul4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rnf149em1flox/Cya mice (Catalog S-CKO-19004) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000062525
NCBI RefSeq
NM_001033135
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Rnf149, or ring finger protein 149, is an E3 ubiquitin ligase. E3 ubiquitin ligases play a crucial role in the ubiquitination process, which tags proteins for degradation or modulates their functions, thus participating in various cellular pathways and biological processes [1,2,3,4,5,6].
In myocardial infarction (MI), Rnf149 is highly expressed in murine and human cardiac macrophages at the early phase. Knockout of Rnf149, transplantation of Rnf149 -/- bone marrow, and bone marrow macrophage-specific Rnf149-knockdown exacerbated cardiac dysfunction in murine MI models. It was found that Rnf149 in infiltrated macrophages restricted inflammation by promoting ubiquitylation-dependent proteasomal degradation of IFNGR1, and loss of IFNGR1 rescued the deleterious effects of Rnf149 deficiency on MI. Also, STAT1 activation induced Rnf149 transcription, creating a feedback control mechanism to fine-tune macrophage-driven inflammation [1].
In lipopolysaccharide/Toll-like receptor 4 (LPS/TLR4) signal transduction, Rnf149 negatively regulates by mediating ubiquitination-induced CD63 degradation, as it can directly interact with CD63 and degrade it through covalent modification [2].
In hepatocellular carcinoma (HCC), Rnf149 is strikingly upregulated in tumor tissues, and overexpression promotes cell proliferation, migration, and invasion via its E3 ubiquitin ligase activity, with DNAJC25 identified as its new substrate [3].
In esophageal squamous cell carcinoma (ESCC), Rnf149 is upregulated and confers cisplatin resistance by interacting with PHLPP2 and inducing its E3 ligase-dependent protein degradation, activating the PI3K/AKT signalling pathway [4].
In conclusion, Rnf149, as an E3 ubiquitin ligase, plays important roles in multiple biological processes and disease conditions. Gene knockout or knockdown studies in mouse models have revealed its functions in cardiac repair post-infarction, immune signal transduction, and cancer progression. These findings highlight the significance of Rnf149 as a potential therapeutic target in relevant diseases such as MI, HCC, and ESCC.
References：
1. Huang, Chun-Kai, Chen, Zhiyong, Zhou, Zhongxing, Yan, Xiaoxiang, Chai, Dajun. 2024. RNF149 Destabilizes IFNGR1 in Macrophages to Favor Postinfarction Cardiac Repair. In Circulation research, 135, 518-536. doi:10.1161/CIRCRESAHA.123.324023. https://pubmed.ncbi.nlm.nih.gov/38989590/
2. Yang, Xiu-An, Wang, Yingying, Gong, Mingyu, Zhang, Xiaoyu, Li, Yan. 2024. RNF149 negatively regulates LPS/TLR4 signal transduction by ubiquitination-mediated CD63 degradation. In Heliyon, 10, e34350. doi:10.1016/j.heliyon.2024.e34350. https://pubmed.ncbi.nlm.nih.gov/39104473/
3. Guo, Zhaoyu, Jiang, Pei, Dong, Qian, Tian, Chunyan, Sun, Aihua. 2023. RNF149 Promotes HCC Progression through Its E3 Ubiquitin Ligase Activity. In Cancers, 15, . doi:10.3390/cancers15215203. https://pubmed.ncbi.nlm.nih.gov/37958377/
4. Zhu, Jinrong, Tang, Jiuren, Wu, Yongqi, Jin, Xin, Zhang, Rongxin. 2023. RNF149 confers cisplatin resistance in esophageal squamous cell carcinoma via destabilization of PHLPP2 and activating PI3K/AKT signalling. In Medical oncology (Northwood, London, England), 40, 290. doi:10.1007/s12032-023-02137-z. https://pubmed.ncbi.nlm.nih.gov/37658961/
5. Legesse, Aster, Kaushansky, Nathali, Braunstein, Ilana, Navon, Ami, Stanhill, Ariel. 2023. The role of RNF149 in the pre-emptive quality control substrate ubiquitination. In Communications biology, 6, 385. doi:10.1038/s42003-023-04763-9. https://pubmed.ncbi.nlm.nih.gov/37031316/
6. Manku, Gurpreet, Kong, Chi-Chon, Culty, Martine. 2022. Role of the Ubiquitin Ligase RNF149 in the Development of Rat Neonatal Gonocytes. In Frontiers in endocrinology, 13, 896507. doi:10.3389/fendo.2022.896507. https://pubmed.ncbi.nlm.nih.gov/35634494/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen