C57BL/6JCya-Slc7a2em1flox/Cya
Common Name:
Slc7a2-flox
Product ID:
S-CKO-19008
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc7a2-flox
Strain ID
CKOCMP-11988-Slc7a2-B6J-VB
Gene Name
Product ID
S-CKO-19008
Gene Alias
20.5; Atrc2; CAT-2; Cat2; Tea
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc7a2em1flox/Cya mice (Catalog S-CKO-19008) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000098816
NCBI RefSeq
NM_001044740
Target Region
Exon 4~6
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Slc7a2, a cationic amino acid transporter, plays crucial roles in multiple biological processes. It is involved in amino acid-related metabolism, such as lysine and arginine metabolism, and is associated with pathways like the PI3K/Akt/NF-κB pathway [3]. It has significance in various physiological functions including cell proliferation and immune regulation [3,6]. Genetic models, especially knockout models, are valuable for studying its functions.
In cancer, loss-of-function studies have revealed diverse roles. In hepatocellular carcinoma, SLC7A2 deficiency promotes cancer progression by enhancing recruitment of myeloid-derived suppressor cells via upregulating CXCL1 through the PI3K/Akt/NF-κB pathway [3]. In ovarian cancer, knockdown of SLC7A2 promotes cell viability, invasion and migration [4]. In NSCLC, SLC7A2 silencing enhances cell proliferation and drug insensitivity while lower SLC7A2 expression is related to less immune infiltrates and worse prognosis [5]. In triple negative breast cancer, low SLC7A2 levels are associated with advanced stages and lymph node metastasis [2]. In glioblastoma stem cells, upregulation of SLC7A2 is part of a lysine catabolism reprogramming that impacts tumour immunity [1]. In pancreatic islets, Slc7a2 knockout mice show decreased arginine-stimulated glucagon and insulin secretion, indicating its role in islet hormone secretion [6].
In conclusion, Slc7a2 is essential for amino acid-related metabolism, cell proliferation, and immune regulation. Knockout mouse models have been instrumental in uncovering its roles in cancer development, progression, and islet function. Understanding Slc7a2 provides insights into disease mechanisms, potentially guiding the development of new therapeutic strategies for cancer and metabolic diseases.
References:
1. Yuan, Huairui, Wu, Xujia, Wu, Qiulian, Snyder, Nathaniel W, Rich, Jeremy N. 2023. Lysine catabolism reprograms tumour immunity through histone crotonylation. In Nature, 617, 818-826. doi:10.1038/s41586-023-06061-0. https://pubmed.ncbi.nlm.nih.gov/37198486/
2. Sun, Yuanyuan, Li, Yaqing, Jiang, Chengying, Liu, Chenying, Song, Yuanming. . SLC7A2-Mediated Lysine Catabolism Inhibits Immunosuppression in Triple Negative Breast Cancer. In Critical reviews in eukaryotic gene expression, 34, 31-43. doi:10.1615/CritRevEukaryotGeneExpr.2024052503. https://pubmed.ncbi.nlm.nih.gov/38842202/
3. Xia, Suhong, Wu, Jingwen, Zhou, Wangdong, Tian, Dean, Liao, Jiazhi. 2021. SLC7A2 deficiency promotes hepatocellular carcinoma progression by enhancing recruitment of myeloid-derived suppressors cells. In Cell death & disease, 12, 570. doi:10.1038/s41419-021-03853-y. https://pubmed.ncbi.nlm.nih.gov/34108444/
4. Sun, Tianshui, Bi, Fangfang, Liu, Zhuonan, Yang, Qing. 2020. SLC7A2 serves as a potential biomarker and therapeutic target for ovarian cancer. In Aging, 12, 13281-13296. doi:10.18632/aging.103433. https://pubmed.ncbi.nlm.nih.gov/32647070/
5. Jiang, Shanshan, Zou, Junrong, Dong, Jianyu, Duan, Xianglong, Li, Wensheng. 2023. Lower SLC7A2 expression is associated with enhanced multidrug resistance, less immune infiltrates and worse prognosis of NSCLC. In Cell communication and signaling : CCS, 21, 9. doi:10.1186/s12964-022-01023-x. https://pubmed.ncbi.nlm.nih.gov/36639771/
6. Spears, Erick, Stanley, Jade E, Shou, Matthew, Powers, Alvin C, Dean, E Danielle. 2023. Pancreatic islet α cell function and proliferation requires the arginine transporter SLC7A2. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.08.10.552656. https://pubmed.ncbi.nlm.nih.gov/37645716/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen