C57BL/6JCya-Atp1b1em1flox/Cya
Common Name:
Atp1b1-flox
Product ID:
S-CKO-19043
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Atp1b1-flox
Strain ID
CKOCMP-11931-Atp1b1-B6J-VB
Gene Name
Product ID
S-CKO-19043
Gene Alias
Atp4b; Atpb; Atpb-1; NKbeta1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atp1b1em1flox/Cya mice (Catalog S-CKO-19043) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027863
NCBI RefSeq
NM_009721
Target Region
Exon 3~4
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
ATP1B1, encoding the Na,K-ATPase β subunit, is a key regulator of the Na+ and K+ electrochemical gradients across the plasma membrane, which is essential for regulating cellular activity. It is involved in various biological processes and is associated with multiple pathways, such as those related to cell adhesion, antiviral innate immunity, and the regulation of cell proliferation, migration, and invasion [2,4,6].
In cancer research, ATP1B1 is overexpressed in diffuse large B-cell lymphoma (DLBCL) cell lines and its expression levels can impact the prognosis of DLBCL patients. Downregulation of ATP1B1 inhibits DLBCL cell proliferation, migration, invasion, and adhesion, and roxithromycin can rescue the higher proliferation ability in ATP1B1-overexpression cells [4]. In cytogenetically normal acute myeloid leukemia (CN-AML), high ATP1B1 expression is associated with shorter overall survival and event-free survival, and it may contribute to leukemogenicity through up-regulation of oncogenes/onco-microRNAs [6]. Rapamycin inhibits the progression of human acute myeloid leukemia by regulating the circ_0094100/miR-217/ATP1B1 axis [8]. In addition, in NRG1 fusion-driven tumors, ATP1B1 is identified as a fusion partner of NRG1, and drugs targeting the related pathways may be potential therapeutic strategies [1,3].
In antiviral innate immunity, ATP1B1 can be induced by DNA and RNA virus infections, inhibit viral replication, and increase the levels of IFNs, IFN-stimulated genes, and inflammatory cytokines by potentiating the ubiquitination of TRAF3 and TRAF6 [2].
In boar sperm, the relative mRNA expression level of ATP1B1 is significantly higher in fresh sperm of the group with good semen freezability, indicating it may be a promising cryotolerance marker [5].
In alveolar epithelial cells, a study identified proteins interacting with ATP1B1, providing new insights into its role in maintaining alveolar epithelial barrier integrity [7].
In astrocytes, the interaction of DCF1 with ATP1B1 impairs astrocyte structural plasticity via the P38 signaling pathway [9].
In Fuchs endothelial corneal dystrophy, intergenic variants are associated with decreased ATP1B1 expression [10].
In conclusion, ATP1B1 is crucial for maintaining normal cellular functions, and its dysregulation is involved in multiple diseases, including various cancers, viral infections, and corneal dystrophy. Research on ATP1B1, especially through studies in disease-relevant models, helps to understand the underlying molecular mechanisms, providing potential diagnostic biomarkers and therapeutic targets for these diseases.
References:
1. Laskin, J, Liu, S V, Tolba, K, Solca, F, Duruisseaux, M. 2020. NRG1 fusion-driven tumors: biology, detection, and the therapeutic role of afatinib and other ErbB-targeting agents. In Annals of oncology : official journal of the European Society for Medical Oncology, 31, 1693-1703. doi:10.1016/j.annonc.2020.08.2335. https://pubmed.ncbi.nlm.nih.gov/32916265/
2. Cao, Wei, Guo, Yifei, Cheng, Zhikui, Liu, Shi, Zhu, Ying. 2021. Inducible ATP1B1 Upregulates Antiviral Innate Immune Responses by the Ubiquitination of TRAF3 and TRAF6. In Journal of immunology (Baltimore, Md. : 1950), 206, 2668-2681. doi:10.4049/jimmunol.2001262. https://pubmed.ncbi.nlm.nih.gov/34011520/
3. Schram, Alison M, Odintsov, Igor, Espinosa-Cotton, Madelyn, Drilon, Alexander, Somwar, Romel. . Zenocutuzumab, a HER2xHER3 Bispecific Antibody, Is Effective Therapy for Tumors Driven by NRG1 Gene Rearrangements. In Cancer discovery, 12, 1233-1247. doi:10.1158/2159-8290.CD-21-1119. https://pubmed.ncbi.nlm.nih.gov/35135829/
4. Zhang, Shuo, Wang, Hongmin, Liu, Aichun. . Identification of ATP1B1, a key copy number driver gene in diffuse large B-cell lymphoma and potential target for drugs. In Annals of translational medicine, 10, 1136. doi:10.21037/atm-22-4709. https://pubmed.ncbi.nlm.nih.gov/36388804/
5. Mańkowska, Anna, Gilun, Przemysław, Zasiadczyk, Łukasz, Sobiech, Przemysław, Fraser, Leyland. 2022. Expression of TXNRD1, HSPA4L and ATP1B1 Genes Associated with the Freezability of Boar Sperm. In International journal of molecular sciences, 23, . doi:10.3390/ijms23169320. https://pubmed.ncbi.nlm.nih.gov/36012584/
6. Shi, Jin-long, Fu, Lin, Ang, Qing, Zhu, Jun, Wang, Wei-dong. . Overexpression of ATP1B1 predicts an adverse prognosis in cytogenetically normal acute myeloid leukemia. In Oncotarget, 7, 2585-95. doi:10.18632/oncotarget.6226. https://pubmed.ncbi.nlm.nih.gov/26506237/
7. Zheng, Yu, Peng, Weiting, Wen, Xupeng, Wan, Qiquan. 2024. Protein interactome analysis of ATP1B1 in alveolar epithelial cells using Co-Immunoprecipitation mass spectrometry and parallel reaction monitoring assay. In Heliyon, 10, e32579. doi:10.1016/j.heliyon.2024.e32579. https://pubmed.ncbi.nlm.nih.gov/38912441/
8. Cao, Jiufang, Huang, Shihua, Li, Xiaoming. 2022. Rapamycin inhibits the progression of human acute myeloid leukemia by regulating the circ_0094100/miR-217/ATP1B1 axis. In Experimental hematology, 112-113, 60-69.e2. doi:10.1016/j.exphem.2022.07.298. https://pubmed.ncbi.nlm.nih.gov/35901982/
9. Wang, Jiao, Zhou, Fangfang, Wang, Dong, Xie, Jiang, Wen, Tieqiao. 2018. Interaction of DCF1 with ATP1B1 induces impairment in astrocyte structural plasticity via the P38 signaling pathway. In Experimental neurology, 302, 214-229. doi:10.1016/j.expneurol.2018.01.007. https://pubmed.ncbi.nlm.nih.gov/29337145/
10. Chakraborty, Maynak, Jandhyam, Harithalakshmi, Basak, Samar Kumar, Das, Sujata, Alone, Debasmita Pankaj. 2023. Intergenic variants, rs1200114 and rs1200108 are genetically associated along with a decreased ATP1B1 expression in Fuchs Endothelial Corneal Dystrophy. In Experimental eye research, 228, 109403. doi:10.1016/j.exer.2023.109403. https://pubmed.ncbi.nlm.nih.gov/36736852/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen