C57BL/6JCya-Hook2em1flox/Cya
Common Name:
Hook2-flox
Product ID:
S-CKO-19052
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Hook2-flox
Strain ID
CKOCMP-170833-Hook2-B6J-VB
Gene Name
Product ID
S-CKO-19052
Gene Alias
A630054I03Rik; mHK2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hook2em1flox/Cya mice (Catalog S-CKO-19052) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064495
NCBI RefSeq
NM_133255
Target Region
Exon 8~10
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Hook2 is an evolutionarily conserved dynein adaptor protein. It plays essential roles in promoting the assembly of highly processive dynein-dynactin motor complexes, and is involved in multiple crucial cellular processes such as mitotic progression, cytokinesis, ciliogenesis, polarized cell migration, and aggresome formation [1-5]. It is also potentially associated with diseases like primary angle closure glaucoma and type 2 diabetes [5,6,7].
In mitosis, Hook2 binds to and promotes dynein-dynactin assembly. During late G2 phase, it mediates dynein-dynactin localization at the nuclear envelope for centrosome anchoring. It also regulates microtubule nucleation at the centrosome independently of its dynein-binding. Hook2-depleted cells show reduced astral microtubules, spindle positioning defects, and cytokinesis failure [1].
In ciliogenesis, Hook2 localizes at the Golgi apparatus and centrosome/basal body. Its depletion disrupts ciliogenesis before ciliary vesicle formation. It interacts with and stabilizes PCM1, and together with Rab8a, regulates an important step in ciliogenesis [2].
Regarding polarized cell migration, Hook2 is an interactor for the aPKC/PAR6α complex, localizing this complex at the centrosome and regulating centrosome orientation [3]. Overexpression of Hook2 promotes aggresome formation, while a dominant-negative form inhibits it, suggesting it contributes to the pericentrosomal localization of aggresomes [4].
In summary, Hook2 is crucial for various cellular functions including mitosis, ciliogenesis, cell migration, and aggresome formation. Its study, especially through gene-knockout models, helps in understanding the underlying mechanisms of these processes. The potential associations with diseases like glaucoma and type 2 diabetes further highlight its significance in disease-related research, providing insights into possible disease mechanisms and potential therapeutic targets.
References:
1. Dwivedi, Devashish, Kumari, Amrita, Rathi, Siddhi, Mylavarapu, Sivaram V S, Sharma, Mahak. 2019. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. In The Journal of cell biology, 218, 871-894. doi:10.1083/jcb.201804183. https://pubmed.ncbi.nlm.nih.gov/30674580/
2. Baron Gaillard, Carole L, Pallesi-Pocachard, Emilie, Massey-Harroche, Dominique, Borg, Jean-Paul, Le Bivic, André. 2011. Hook2 is involved in the morphogenesis of the primary cilium. In Molecular biology of the cell, 22, 4549-62. doi:10.1091/mbc.E11-05-0405. https://pubmed.ncbi.nlm.nih.gov/21998199/
3. Pallesi-Pocachard, Emilie, Bazellieres, Elsa, Viallat-Lieutaud, Annelise, Le Bivic, André, Massey-Harroche, Dominique. 2016. Hook2, a microtubule-binding protein, interacts with Par6α and controls centrosome orientation during polarized cell migration. In Scientific reports, 6, 33259. doi:10.1038/srep33259. https://pubmed.ncbi.nlm.nih.gov/27624926/
4. Szebenyi, Györgyi, Wigley, W Christian, Hall, Branden, Thomas, Philip, Krämer, Helmut. 2007. Hook2 contributes to aggresome formation. In BMC cell biology, 8, 19. doi:. https://pubmed.ncbi.nlm.nih.gov/17540036/
5. Qiao, Chunyan, Jia, Hongyan, Zhang, Hui, Cao, Kai, Hu, Jianping. 2020. Coding Variants in HOOK2 and GTPBP3 May Contribute to Risk of Primary Angle Closure Glaucoma. In DNA and cell biology, 39, 949-957. doi:10.1089/dna.2019.5079. https://pubmed.ncbi.nlm.nih.gov/32397755/
6. Nadiger, Nikhil, Veed, Jyothisha Kana, Chinya Nataraj, Priyanka, Mukhopadhyay, Arpita. 2024. DNA methylation and type 2 diabetes: a systematic review. In Clinical epigenetics, 16, 67. doi:10.1186/s13148-024-01670-6. https://pubmed.ncbi.nlm.nih.gov/38755631/
7. Rodríguez-Rodero, Sandra, Menéndez-Torre, Edelmiro, Fernández-Bayón, Gustavo, Fraga, Mario F, Delgado-Álvarez, Elías. 2017. Altered intragenic DNA methylation of HOOK2 gene in adipose tissue from individuals with obesity and type 2 diabetes. In PloS one, 12, e0189153. doi:10.1371/journal.pone.0189153. https://pubmed.ncbi.nlm.nih.gov/29228058/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen