C57BL/6JCya-Dcaf11em1flox/Cya
Common Name:
Dcaf11-flox
Product ID:
S-CKO-19073
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dcaf11-flox
Strain ID
CKOCMP-28199-Dcaf11-B6J-VB
Gene Name
Product ID
S-CKO-19073
Gene Alias
0710008A13Rik; D14Ucla1; Dacf11; GLO14; Wdr23
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dcaf11em1flox/Cya mice (Catalog S-CKO-19073) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000072530
NCBI RefSeq
NM_001199009
Target Region
Exon 3~11
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Dcaf11, also known as Ddb1-and Cul4-associated factor 11, is an E3 ligase substrate adaptor that plays crucial roles in multiple biological processes. It is involved in pathways such as ubiquitin-proteasome-mediated proteolysis, which is essential for regulating protein levels in cells. This gene is important for maintaining chromosome stability, cell cycle progression, and redox homeostasis [1,2,3,4].
In early embryos and embryonic stem cells, deletion of Dcaf11 leads to reduced telomere sister-chromatid exchange and impairs telomere lengthening. Dcaf11-deficient mice exhibit gradual telomere erosion over successive generations, and hematopoietic stem cell activity is greatly compromised. Mechanistically, Dcaf11 targets Kap1 for ubiquitination-mediated degradation, activating the Zscan4 downstream enhancer and removing heterochromatic H3K9me3 at telomere/subtelomere regions [1]. In human osteosarcoma cells, knocking down components of the CRL4BDCAF11 complex (including DCAF11) attenuates the ubiquitination level of p21Cip1, inhibits cell proliferation, leads to cell cycle arrest at S phase, and decreases colony formation rate [3].
In conclusion, Dcaf11 is vital for telomere elongation in early embryos and stem cells, as well as for regulating cell cycle progression in osteosarcoma cells. Studies using Dcaf11-deficient mouse models have provided valuable insights into its role in maintaining chromosome stability and cell-cycle-related diseases, helping to understand the underlying molecular mechanisms and potentially paving the way for new therapeutic strategies.
References:
1. Le, Rongrong, Huang, Yixin, Zhang, Yanping, Ju, Zhenyu, Gao, Shaorong. 2020. Dcaf11 activates Zscan4-mediated alternative telomere lengthening in early embryos and embryonic stem cells. In Cell stem cell, 28, 732-747.e9. doi:10.1016/j.stem.2020.11.018. https://pubmed.ncbi.nlm.nih.gov/33357405/
2. Wang, Kehui, Liu, Yuting, Yu, Zhouliang, Xu, Rui-Ming, Li, Guohong. . Phosphorylation at Ser68 facilitates DCAF11-mediated ubiquitination and degradation of CENP-A during the cell cycle. In Cell reports, 37, 109987. doi:10.1016/j.celrep.2021.109987. https://pubmed.ncbi.nlm.nih.gov/34758320/
3. Chen, Zhi, Wang, Kun, Hou, Canglong, Zhang, Caiguo, Shen, Hongxing. 2017. CRL4BDCAF11 E3 ligase targets p21 for degradation to control cell cycle progression in human osteosarcoma cells. In Scientific reports, 7, 1175. doi:10.1038/s41598-017-01344-9. https://pubmed.ncbi.nlm.nih.gov/28446751/
4. Hayes, John D, Dayalan Naidu, Sharadha, Dinkova-Kostova, Albena T. 2025. Regulating Nrf2 activity: ubiquitin ligases and signaling molecules in redox homeostasis. In Trends in biochemical sciences, 50, 179-205. doi:10.1016/j.tibs.2024.12.010. https://pubmed.ncbi.nlm.nih.gov/39875264/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen