C57BL/6JCya-Sltmem1flox/Cya
Common Name:
Sltm-flox
Product ID:
S-CKO-19080
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Sltm-flox
Strain ID
CKOCMP-66660-Sltm-B6J-VB
Gene Name
Product ID
S-CKO-19080
Gene Alias
5730455C01Rik; 5730555F13Rik; 9130215G10Rik; Met
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sltmem1flox/Cya mice (Catalog S-CKO-19080) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000049263
NCBI RefSeq
NM_025690
Target Region
Exon 6~8
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Sltm, also known as SAFB-like transcription modulator, is a chromatin-associated protein with DNA and RNA binding capacities. It belongs to the SAFB family and is involved in multiple biological processes. It has been implicated in pathways such as the Notch pathway, and is important for the transcriptional regulation in the Sonic hedgehog (SHH) signaling pathway [2,3].
In a gene-based rare variant association study, rare heterozygous loss-of-function of Sltm was found to increase adult body mass index (BMI) in men, suggesting its role in the genetic regulation of obesity [1]. In hepatocellular carcinoma, hypoxia-induced DTL promotes the ubiquitination and degradation of Sltm, which in turn activates the Notch pathway, promoting cancer cell proliferation, metastasis, and sorafenib resistance [2]. In an in vivo study, Sltm deletion through Nuclease technology-mediated gene editing in mice caused perinatal lethality and SHH-related abnormal ventral neural tube phenotypes, indicating its crucial role in embryonic development [3].
In conclusion, Sltm plays essential roles in processes like obesity-related genetic regulation, cancer development, and embryonic development. The use of gene knockout models, such as the Nuclease technology-mediated Sltm deletion in mice, has been instrumental in revealing these functions, providing insights into potential disease mechanisms and therapeutic targets in areas like obesity and hepatocellular carcinoma [1,2,3].
References:
1. Kaisinger, Lena R, Kentistou, Katherine A, Stankovic, Stasa, Ong, Ken K, Perry, John R B. 2023. Large-scale exome sequence analysis identifies sex- and age-specific determinants of obesity. In Cell genomics, 3, 100362. doi:10.1016/j.xgen.2023.100362. https://pubmed.ncbi.nlm.nih.gov/37601970/
2. Chen, Zi-Xiong, Mu, Mao-Yuan, Yang, Guang, Huang, Bi-Jun, Gao, Fei. 2024. Hypoxia-induced DTL promotes the proliferation, metastasis, and sorafenib resistance of hepatocellular carcinoma through ubiquitin-mediated degradation of SLTM and subsequent Notch pathway activation. In Cell death & disease, 15, 734. doi:10.1038/s41419-024-07089-4. https://pubmed.ncbi.nlm.nih.gov/39384740/
3. Zhang, Zilai, Zhan, Xiaoming, Kim, Bongwoo, Wu, Jiang. 2019. A proteomic approach identifies SAFB-like transcription modulator (SLTM) as a bidirectional regulator of GLI family zinc finger transcription factors. In The Journal of biological chemistry, 294, 5549-5561. doi:10.1074/jbc.RA118.007018. https://pubmed.ncbi.nlm.nih.gov/30782847/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen