C57BL/6JCya-P2rx5em1flox/Cya
Common Name:
P2rx5-flox
Product ID:
S-CKO-19084
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
P2rx5-flox
Strain ID
CKOCMP-94045-P2rx5-B6J-VB
Gene Name
Product ID
S-CKO-19084
Gene Alias
P2X5
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-P2rx5em1flox/Cya mice (Catalog S-CKO-19084) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006104
NCBI RefSeq
NM_033321
Target Region
Exon 2~10
Size of Effective Region
~4.0 kb
Detailed Document
Overview of Gene Research
P2rx5, a member of the P2X family of ligand-gated cation channels, mediates signaling in response to extracellular ATP [2]. It has been associated with multiple biological processes and diseases. P2rx5 is expressed in various cell types, including neurons, muscle cells, leukocytes, and adipocytes, and may be involved in the regulation of purinergic receptor signaling pathways [2,3,4,5].
In brown adipocyte research, P2rx5 knockout male mice showed reduced brown adipocyte differentiation in vitro and browning in vivo, indicating its role in mediating brown adipocyte differentiation and function, which could potentially be targeted for adipose tissue pathology and metabolic diseases [1]. In experimental periodontitis, P2rx5 -/- mice had decreased bone loss compared to wild-type and P2rx7 -/- mice, suggesting that P2X5 may regulate bone loss related to periodontitis and could be a novel therapeutic target [5].
In conclusion, P2rx5 plays essential roles in brown adipocyte differentiation and energy homeostasis, as well as in regulating bone loss in periodontitis. The use of gene knockout mouse models has been crucial in revealing these functions, providing potential therapeutic directions for metabolic diseases and periodontitis [1,5].
References：
1. Razzoli, Maria, McGonigle, Seth, Sahu, Bhavani Shankar, Frontini, Andrea, Bartolomucci, Alessandro. 2024. A key role for P2RX5 in brown adipocyte differentiation and energy homeostasis. In Adipocyte, 13, 2421745. doi:10.1080/21623945.2024.2421745. https://pubmed.ncbi.nlm.nih.gov/39484996/
2. Abramowski, Pierre, Ogrodowczyk, Christoph, Martin, Roland, Pongs, Olaf. 2014. A truncation variant of the cation channel P2RX5 is upregulated during T cell activation. In PloS one, 9, e104692. doi:10.1371/journal.pone.0104692. https://pubmed.ncbi.nlm.nih.gov/25181038/
3. Alnafisah, Rawan, Lundh, Anna, Asah, Sophie M, McCullumsmith, Robert E, O'Donovan, Sinead M. 2022. Altered purinergic receptor expression in the frontal cortex in schizophrenia. In Schizophrenia (Heidelberg, Germany), 8, 96. doi:10.1038/s41537-022-00312-1. https://pubmed.ncbi.nlm.nih.gov/36376358/
4. Grigoraş, Adriana, Amalinei, Cornelia, Balan, Raluca Anca, Lozneanu, Ludmila, Căruntu, Irina-Draga. 2018. Adipocytes spectrum - From homeostasia to obesity and its associated pathology. In Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, 219, 102-120. doi:10.1016/j.aanat.2018.06.004. https://pubmed.ncbi.nlm.nih.gov/30049662/
5. Kim, Hyunsoo, Kajikawa, Tetsuhiro, Walsh, Matthew C, Hajishengallis, George, Choi, Yongwon. . The purinergic receptor P2X5 contributes to bone loss in experimental periodontitis. In BMB reports, 51, 468-473. doi:. https://pubmed.ncbi.nlm.nih.gov/30103845/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen