C57BL/6JCya-Prkg1em1flox/Cya
Common Name:
Prkg1-flox
Product ID:
S-CKO-19115
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Prkg1-flox
Strain ID
CKOCMP-19091-Prkg1-B6J-VB
Gene Name
Product ID
S-CKO-19115
Gene Alias
CGKI; Gm19690; Prkg1b; Prkgr1b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prkg1em1flox/Cya mice (Catalog S-CKO-19115) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000073581
NCBI RefSeq
NM_011160
Target Region
Exon 10
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Prkg1, encoding cGMP-dependent protein kinase type I, is a key molecule in the cGMP signaling pathway. It is involved in multiple biological processes, with its functions including regulating cell-related activities such as contraction in aortic smooth muscle cells, and its role in pathways like NO/cGMP/PKG is crucial for various physiological functions [3,5].
In mice, genetic deletion of Prkg1 led to a greater vulnerability to and less recovery from noise-induced hearing loss (NIHL), suggesting an endogenous protective cGMP-Prkg1 signaling pathway in cochlear hair cells and hearing function [4].
In the dorsomedial hypothalamus (DMH) of mice, within DMHPpp1r17 neurons, Prkg1-regulated phosphorylation and translocation of Ppp1r17 affect gene expression regulating synaptic function, and DMH-specific Prkg1 knockdown can ameliorate age-associated dysfunction in white adipose tissue (WAT), increase physical activity, and extend lifespan [2]. Also, vericiguat, used to treat heart failure, can up-regulate Prkg1, which in turn activates PINK1 and inhibits the STING/IRF3 pathway, thus alleviating doxorubicin-induced cardiotoxicity in mice [1].
In conclusion, Prkg1 is essential in the cGMP signaling pathway and plays important roles in various biological processes and disease conditions. Studies using Prkg1-related genetic mouse models have revealed its significance in NIHL, aging-related processes, and cardiotoxicity, providing insights into the underlying molecular mechanisms and potential therapeutic targets.
References:
1. Zeng, Xianghui, Zhang, Hao, Xu, Tianyu, Xu, Dingli, Ren, Hao. 2024. Vericiguat attenuates doxorubicin-induced cardiotoxicity through the PRKG1/PINK1/STING axis. In Translational research : the journal of laboratory and clinical medicine, 273, 90-103. doi:10.1016/j.trsl.2024.07.005. https://pubmed.ncbi.nlm.nih.gov/39059761/
2. Tokizane, Kyohei, Brace, Cynthia S, Imai, Shin-Ichiro. 2024. DMHPpp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication. In Cell metabolism, 36, 377-392.e11. doi:10.1016/j.cmet.2023.12.011. https://pubmed.ncbi.nlm.nih.gov/38194970/
3. Micale, Lucia, Fusco, Carmela, Nardella, Grazia, Perri, Francesco, Latiano, Anna. 2022. Downexpression of miR-200c-3p Contributes to Achalasia Disease by Targeting the PRKG1 Gene. In International journal of molecular sciences, 24, . doi:10.3390/ijms24010668. https://pubmed.ncbi.nlm.nih.gov/36614110/
4. Jaumann, Mirko, Dettling, Juliane, Gubelt, Martin, Knipper, Marlies, Rüttiger, Lukas. 2012. cGMP-Prkg1 signaling and Pde5 inhibition shelter cochlear hair cells and hearing function. In Nature medicine, 18, 252-9. doi:10.1038/nm.2634. https://pubmed.ncbi.nlm.nih.gov/22270721/
5. Gago-Díaz, Marina, Blanco-Verea, Alejandro, Teixidó, Gisela, Evangelista, Artur, Brion, María. 2016. PRKG1 and genetic diagnosis of early-onset thoracic aortic disease. In European journal of clinical investigation, 46, 787-94. doi:10.1111/eci.12662. https://pubmed.ncbi.nlm.nih.gov/27442293/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen