C57BL/6JCya-Coq3em1flox/Cya
Common Name:
Coq3-flox
Product ID:
S-CKO-19129
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Coq3-flox
Strain ID
CKOCMP-230027-Coq3-B6J-VB
Gene Name
Product ID
S-CKO-19129
Gene Alias
4732433J24
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Coq3em1flox/Cya mice (Catalog S-CKO-19129) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029909
NCBI RefSeq
NM_172687
Target Region
Exon 4~5
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Coq3, a gene encoding 3,4-dihydroxy-5-polyprenylbenzoate methyltransferase, is crucial for coenzyme Q (Q) biosynthesis [4,5,7,9]. Q is a redox-active lipid essential for aerobic respiration in eukaryotes, and Coq3-catalyzed O-methylation steps are key in its biosynthetic pathway [1,2,5,6,8,9]. Coq3 is part of a polypeptide complex in yeast mitochondria for Q biosynthesis, with Coq3 and Coq4 co-migrating as high-molecular-mass complexes [1]. It is also a membrane-binding protein, with human and yeast Coq3 preferring liposomes enriched with cardiolipin, a mitochondrial membrane lipid [2].
In Coq3-related functional studies, Rtn4ip1-knockout myoblasts showed decreased CoQ9 levels, suggesting that RTN4IP1, an NADPH oxidoreductase, is essential for CoQ biosynthesis by regulating Coq3 O-methylation activity [3]. In yeast, Coq3 null mutants can be rescued by expressing the human Coq3 construct or the E. coli UbiG polypeptide with a mitochondrial leader sequence, restoring Q biosynthesis and growth on non-fermentable carbon sources [5,6].
In conclusion, Coq3 is essential for coenzyme Q biosynthesis, playing a key role in aerobic respiration. Functional studies, including knockout models in myoblasts and yeast mutants, have revealed its role in CoQ synthesis. Understanding Coq3 can provide insights into mitochondrial function and potentially related disease conditions associated with Q deficiencies [3,5,6].
References：
1. Marbois, Beth, Gin, Peter, Faull, Kym F, Shepherd, Jennifer N, Clarke, Catherine F. 2005. Coq3 and Coq4 define a polypeptide complex in yeast mitochondria for the biosynthesis of coenzyme Q. In The Journal of biological chemistry, 280, 20231-8. doi:. https://pubmed.ncbi.nlm.nih.gov/15792955/
2. Zhu, Yuwei, Wu, Bo, Zhang, Xu, Wang, Junfeng, Teng, Maikun. 2015. Structural and biochemical studies reveal UbiG/Coq3 as a class of novel membrane-binding proteins. In The Biochemical journal, 470, 105-14. doi:10.1042/BJ20150329. https://pubmed.ncbi.nlm.nih.gov/26251450/
3. Park, Isaac, Kim, Kwang-Eun, Kim, Jeesoo, Suh, Jae Myoung, Rhee, Hyun-Woo. 2023. Mitochondrial matrix RTN4IP1/OPA10 is an oxidoreductase for coenzyme Q synthesis. In Nature chemical biology, 20, 221-233. doi:10.1038/s41589-023-01452-w. https://pubmed.ncbi.nlm.nih.gov/37884807/
4. Marbois, B N, Xia, Y R, Lusis, A J, Clarke, C F. . Ubiquinone biosynthesis in eukaryotic cells: tissue distribution of mRNA encoding 3,4-dihydroxy-5-polyprenylbenzoate methyltransferase in the rat and mapping of the COQ3 gene to mouse chromosome 4. In Archives of biochemistry and biophysics, 313, 83-8. doi:. https://pubmed.ncbi.nlm.nih.gov/8053692/
5. Jonassen, T, Clarke, C F. . Isolation and functional expression of human COQ3, a gene encoding a methyltransferase required for ubiquinone biosynthesis. In The Journal of biological chemistry, 275, 12381-7. doi:. https://pubmed.ncbi.nlm.nih.gov/10777520/
6. Hsu, A Y, Poon, W W, Shepherd, J A, Myles, D C, Clarke, C F. . Complementation of coq3 mutant yeast by mitochondrial targeting of the Escherichia coli UbiG polypeptide: evidence that UbiG catalyzes both O-methylation steps in ubiquinone biosynthesis. In Biochemistry, 35, 9797-806. doi:. https://pubmed.ncbi.nlm.nih.gov/8703953/
7. Clarke, C F, Williams, W, Teruya, J H. . Ubiquinone biosynthesis in Saccharomyces cerevisiae. Isolation and sequence of COQ3, the 3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase gene. In The Journal of biological chemistry, 266, 16636-44. doi:. https://pubmed.ncbi.nlm.nih.gov/1885593/
8. Poon, W W, Barkovich, R J, Hsu, A Y, Myles, D C, Clarke, C F. . Yeast and rat Coq3 and Escherichia coli UbiG polypeptides catalyze both O-methyltransferase steps in coenzyme Q biosynthesis. In The Journal of biological chemistry, 274, 21665-72. doi:. https://pubmed.ncbi.nlm.nih.gov/10419476/
9. Avelange-Macherel, M H, Joyard, J. . Cloning and functional expression of AtCOQ3, the Arabidopsis homologue of the yeast COQ3 gene, encoding a methyltransferase from plant mitochondria involved in ubiquinone biosynthesis. In The Plant journal : for cell and molecular biology, 14, 203-13. doi:. https://pubmed.ncbi.nlm.nih.gov/9628017/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen