C57BL/6JCya-Mir125b-1em1flox/Cya
Common Name:
Mir125b-1-flox
Product ID:
S-CKO-19135
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mir125b-1-flox
Strain ID
CKOCMP-387236-Mir125b-1-B6J-VA
Gene Name
Product ID
S-CKO-19135
Gene Alias
Mirn125b; Mirn125b-1; mir-125b-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mir125b-1em1flox/Cya mice (Catalog S-CKO-19135) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000175613
NCBI RefSeq
NR_029822
Target Region
Exon 1
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Mir125b-1 is a microRNA gene. MicroRNAs are crucial regulators in various biological processes, often involved in post-transcriptional regulation of gene expression by binding to target mRNAs [4].
In the human brain, Mir125b-1 is not imprinted. In mice, it is highly expressed in the brain but down-regulated during brain development. It shows preferential expression in the olfactory bulb, thalamus, and hypothalamus, and is enriched in GABAergic neurons, especially somatostatin-expressing GABAergic neurons [1]. Also, its methylation level is significantly correlated with the progression of metastatic clear cell renal cell carcinoma, including late stages, large tumor size, metastasis to lymph nodes and/or distant organs, and loss of differentiation in the tumor [2]. In breast cancer, increased methylation of Mir125b-1 is observed in tumors compared to normal tissues, and it is associated with lymph node metastasis, which can be used to develop a marker system for predicting breast cancer metastasis [3,8]. In non-small cell lung cancer, a significantly high level of its methylation is found in adenocarcinoma and squamous cell lung cancer compared to adjacent normal lung tissue [5]. In ovarian carcinoma, it is hypermethylated at the early stages [7]. And in kidney cancer, its increased methylation levels are associated with the development of distant metastases [6].
In summary, Mir125b-1 is involved in the development and progression of multiple cancers, including renal, breast, lung, and ovarian cancers, through methylation-related mechanisms. In the brain, it shows specific spatiotemporal expression patterns during development. Studies on Mir125b-1, especially those related to its methylation and expression in disease models, help in understanding the pathogenesis of these diseases and may provide potential biomarkers for diagnosis and prognosis.
References:
1. Hou, Kuan-Chu, Tsai, Meng-Han, Akbarian, Schahram, Huang, Hsien-Sung. 2023. Mir125b-1 is Not Imprinted in Human Brain and Shows Developmental Expression Changes in Mouse Brain. In Neuroscience, 529, 99-106. doi:10.1016/j.neuroscience.2023.08.014. https://pubmed.ncbi.nlm.nih.gov/37598835/
2. Ivanova, N A, Burdennyi, A M, Lukina, S S, Braga, E A, Kushlinskii, N E. 2023. The Role of Methylation of a Group of microRNA Genes in the Pathogenesis of Metastatic Renal Cell Carcinoma. In Bulletin of experimental biology and medicine, 175, 249-253. doi:10.1007/s10517-023-05844-9. https://pubmed.ncbi.nlm.nih.gov/37466853/
3. Burdennyy, A M, Filippova, E A, Khodyrev, D S, Loginov, V I, Braga, E A. 2021. Optimized Marker System for Early Diagnosis of Breast Cancer. In Bulletin of experimental biology and medicine, 172, 57-62. doi:10.1007/s10517-021-05331-z. https://pubmed.ncbi.nlm.nih.gov/34791555/
4. Kim, Kyu-Han, Bin, Bum-Ho, Kim, Juewon, Cho, Eun-Gyung, Lee, Tae Ryong. 2013. Novel inhibitory function of miR-125b in melanogenesis. In Pigment cell & melanoma research, 27, 140-4. doi:10.1111/pcmr.12179. https://pubmed.ncbi.nlm.nih.gov/24118912/
5. Gubenko, M S, Loginov, V I, Burdennyy, A M, Kazubskaya, T P, Pertsov, S S. 2023. Changes in the Level of Methylation of a Group of microRNA Genes as a Factor in the Development and Progression of Non-Small Cell Lung Cancer. In Bulletin of experimental biology and medicine, 174, 254-258. doi:10.1007/s10517-023-05684-7. https://pubmed.ncbi.nlm.nih.gov/36598670/
6. Apanovich, Natalya, Matveev, Alexey, Ivanova, Natalia, Braga, Eleonora, Alimov, Andrei. 2023. Prediction of Distant Metastases in Patients with Kidney Cancer Based on Gene Expression and Methylation Analysis. In Diagnostics (Basel, Switzerland), 13, . doi:10.3390/diagnostics13132289. https://pubmed.ncbi.nlm.nih.gov/37443682/
7. Loginov, Vitaly I, Pronina, Irina V, Filippova, Elena A, Dmitriev, Alexey A, Braga, Eleonora A. 2022. Aberrant Methylation of 20 miRNA Genes Specifically Involved in Various Steps of Ovarian Carcinoma Spread: From Primary Tumors to Peritoneal Macroscopic Metastases. In International journal of molecular sciences, 23, . doi:10.3390/ijms23031300. https://pubmed.ncbi.nlm.nih.gov/35163224/
8. Loginov, V I, Burdennyy, A M, Filippova, E A, Khodyrev, D S, Braga, E A. 2021. Aberrant Methylation of 21 MicroRNA Genes in Breast Cancer: Sets of Genes Associated with Progression and a System of Markers for Predicting Metastasis. In Bulletin of experimental biology and medicine, 172, 67-71. doi:10.1007/s10517-021-05333-x. https://pubmed.ncbi.nlm.nih.gov/34792716/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen