C57BL/6JCya-Brca1em1flox/Cya
Common Name:
Brca1-flox
Product ID:
S-CKO-19155
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Brca1-flox
Strain ID
CKOCMP-12189-Brca1-B6J-VB
Gene Name
Product ID
S-CKO-19155
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Brca1em1flox/Cya mice (Catalog S-CKO-19155) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000017290
NCBI RefSeq
NM_009764
Target Region
Exon 5~6
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
BRCA1, short for Breast Cancer 1 protein, is a crucial tumor suppressor gene [1,2,3]. It is involved in fundamental cellular functions such as DNA repair, transcriptional regulation, and cell cycle control in response to DNA damage [2]. BRCA1 is essential for maintaining genome integrity in all cells, and its reduced expression due to mutations or epigenetic inactivation can lead to impaired mammary gland differentiation and an increased risk of breast cancer development [1].
Mutations in the BRCA1 gene predispose individuals to breast and ovarian cancers, yet it remains unclear why other cancer types are not as commonly associated [1,2,5]. In ovarian cancer, BRCA1 germline mutations cause hereditary cases, and its epigenetic inactivation contributes to sporadic cases. Evidence suggests BRCA1 may be a biomarker for response to platinum-based chemotherapy in ovarian cancer, with BRCA1 deficiency predicting enhanced response, while loss of its function may reduce response to antimicrotubule-based chemotherapy [6]. For breast cancer, risk-reducing mastectomy in women with BRCA1 pathogenic variants can lower the risk of breast cancer and the probability of dying from it [4].
In conclusion, BRCA1 is vital for maintaining genomic stability through its role in DNA repair and other cellular processes. Its malfunction is strongly linked to breast and ovarian cancers. Studies, including those on individuals with BRCA1 mutations, have provided insights into the role of BRCA1 in these cancers, which may guide preventive and treatment strategies for these diseases.
References:
1. Romagnolo, Alberto P G, Romagnolo, Donato F, Selmin, Ornella I. . BRCA1 as target for breast cancer prevention and therapy. In Anti-cancer agents in medicinal chemistry, 15, 4-14. doi:. https://pubmed.ncbi.nlm.nih.gov/25329591/
2. Yoshida, Kiyotsugu, Miki, Yoshio. . Role of BRCA1 and BRCA2 as regulators of DNA repair, transcription, and cell cycle in response to DNA damage. In Cancer science, 95, 866-71. doi:. https://pubmed.ncbi.nlm.nih.gov/15546503/
3. Werner, Haim. 2022. BRCA1: An Endocrine and Metabolic Regulator. In Frontiers in endocrinology, 13, 844575. doi:10.3389/fendo.2022.844575. https://pubmed.ncbi.nlm.nih.gov/35432218/
4. Metcalfe, Kelly, Huzarski, Tomasz, Gronwald, Jacek, Lubinski, Jan, Narod, Steven A. 2023. Risk-reducing mastectomy and breast cancer mortality in women with a BRCA1 or BRCA2 pathogenic variant: an international analysis. In British journal of cancer, 130, 269-274. doi:10.1038/s41416-023-02503-8. https://pubmed.ncbi.nlm.nih.gov/38030749/
5. Sedic, Maja, Kuperwasser, Charlotte. . BRCA1-hapoinsufficiency: Unraveling the molecular and cellular basis for tissue-specific cancer. In Cell cycle (Georgetown, Tex.), 15, 621-7. doi:10.1080/15384101.2016.1141841. https://pubmed.ncbi.nlm.nih.gov/26822887/
6. Quinn, Jennifer E, Carser, Judith E, James, Colin R, Kennedy, Richard D, Harkin, D Paul. 2009. BRCA1 and implications for response to chemotherapy in ovarian cancer. In Gynecologic oncology, 113, 134-42. doi:10.1016/j.ygyno.2008.12.015. https://pubmed.ncbi.nlm.nih.gov/19168207/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen