C57BL/6JCya-P4ha2em1flox/Cya
Common Name
P4ha2-flox
Product ID
S-CKO-19157
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-18452-P4ha2-B6J-VB
Status
When using this mouse strain in a publication, please cite “P4ha2-flox Mouse (Catalog S-CKO-19157) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
P4ha2-flox
Strain ID
CKOCMP-18452-P4ha2-B6J-VB
Gene Name
Product ID
S-CKO-19157
Gene Alias
P4hl
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000019050
NCBI RefSeq
NM_011031
Target Region
Exon 7~8
Size of Effective Region
~2.6 kb
Overview of Gene Research
P4ha2, a subunit of prolyl-4-hydroxylases, contains a substrate binding and catalyzation domain and is a key enzyme in collagen synthesis. It is involved in multiple biological processes and signaling pathways, such as the Hippo, PI3K/AKT, and Hedgehog signaling pathways, and is of great biological importance in various physiological and pathological conditions [1,2,3,4,5]. Genetic models, especially gene knockout mouse models, are valuable for studying its functions.
In P4ha2-/-mice injured by DDC, there was a significantly reduced level of ductular reaction and fibrosis compared with controls in the DDC-induced chronic cholestasis. Also, decreased liver fibrosis and ductular reaction were observed in P4ha2-/-/MDR2-/-mice compared with MDR2-/-mice. Cholangiocytes isolated from P4ha2-/-/MDR2-/-mice displayed a higher level of YAP phosphorylation, resulting in cholangiocytes proliferation inhibition. This shows that P4ha2 promotes hepatic ductular reaction and biliary fibrosis by regulating the SAV1-mediated Hippo signaling pathway, indicating its potential as a therapeutic target for primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC) [1].
In conclusion, P4ha2 plays crucial roles in processes like collagen synthesis and regulation of multiple signaling pathways. The study of P4ha2 knockout mouse models has revealed its significant impact on biliary fibrosis and related cholestatic liver diseases, providing insights into potential therapeutic strategies for PBC and PSC.
References：
1. Zhang, Jun, Lyu, Zhuwan, Li, Bo, Xiao, Xiao, Ma, Xiong. 2023. P4HA2 induces hepatic ductular reaction and biliary fibrosis in chronic cholestatic liver diseases. In Hepatology (Baltimore, Md.), 78, 10-25. doi:10.1097/HEP.0000000000000317. https://pubmed.ncbi.nlm.nih.gov/36799463/
2. Li, Quanfu, Liu, Yiyang, Wu, Jingxian, Dang, Yongjun, Jiang, Wei. 2024. P4HA2 hydroxylates SUFU to regulate the paracrine Hedgehog signaling and promote B-cell lymphoma progression. In Leukemia, 38, 1751-1763. doi:10.1038/s41375-024-02313-8. https://pubmed.ncbi.nlm.nih.gov/38909089/
3. Chi, Zengpeng, Wang, Qimin, Wang, Xin, Zheng, Jiawei, Chen, Zhenggang. 2024. P4HA2 promotes proliferation, invasion, and metastasis through regulation of the PI3K/AKT signaling pathway in oral squamous cell carcinoma. In Scientific reports, 14, 15023. doi:10.1038/s41598-024-64264-5. https://pubmed.ncbi.nlm.nih.gov/38951593/
4. Lin, Jing, Jiang, Lei, Wang, Xiaogang, Wu, Xiaojun, Qiu, GuanZhong. 2021. P4HA2 Promotes Epithelial-to-Mesenchymal Transition and Glioma Malignancy through the Collagen-Dependent PI3K/AKT Pathway. In Journal of oncology, 2021, 1406853. doi:10.1155/2021/1406853. https://pubmed.ncbi.nlm.nih.gov/34434233/
5. Wu, Yan-Ling, Liu, Wan, Zhao, Tingting, Jin, Jing. 2024. P4HA2 contributes to head and neck squamous cell carcinoma progression and EMT through PI3K/AKT signaling pathway. In Medical oncology (Northwood, London, England), 41, 163. doi:10.1007/s12032-024-02358-w. https://pubmed.ncbi.nlm.nih.gov/38777998/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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