C57BL/6JCya-Mvpem1flox/Cya
Common Name:
Mvp-flox
Product ID:
S-CKO-19164
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mvp-flox
Strain ID
CKOCMP-78388-Mvp-B6J-VB
Gene Name
Product ID
S-CKO-19164
Gene Alias
2310009M24Rik; LRP; VAULT1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mvpem1flox/Cya mice (Catalog S-CKO-19164) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000165096
NCBI RefSeq
NM_080638
Target Region
Exon 2~3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
MVP, also known as major vault protein, is a crucial protein involved in various biological processes, especially those related to macrophages and bone homeostasis. In macrophages, it is associated with the JAK2-STAT6 pathway [1]. In bone, it is related to osteoclastogenesis and the calcineurin-NFATc1 pathway [2]. It also has a significant impact on bone remodeling and immune-related processes in the body. Genetic models, such as KO and CKO mouse models, have been instrumental in studying its functions.
In macrophage-related studies, myeloid-specific MVP gene knockout (MacKO) mice showed impaired fracture repair due to macrophages' inability to transition from a pro-inflammatory to an anti-inflammatory phenotype. This led to enhanced osteoclastic differentiation and impaired osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) [1].
In bone-related investigations, total body (Mvp-/ -) and monocyte-specific (Mvpf/fLyz2-Cre) MVP gene knockout mice exhibited osteoporosis-like phenotypes. MVP-deficiency enhanced calcineurin-NFATc1 signaling, promoting osteoclastogenesis and bone resorption [2]. Also, conditional knockout of MVP in monocyte lineage (Mvpf/fLyz2-Cre mice) inhibited osteoclast apoptosis in osteoporosis contexts of aging and estrogen deficiency [3].
In conclusion, MVP plays essential roles in macrophage-mediated inflammation during fracture repair and in regulating osteoclastogenesis and osteoclast apoptosis, thereby influencing bone homeostasis. The use of MVP KO/CKO mouse models has significantly contributed to understanding its functions in these disease-relevant biological processes, which may provide potential therapeutic targets for fracture treatment and osteoporosis [1,2,3].
References:
1. Yang, Yan, Zhao, Na, Wang, Ruobing, Ben, Jingjing, Ma, Junqing. 2023. Macrophage MVP regulates fracture repair by promoting M2 polarization via JAK2-STAT6 pathway. In International immunopharmacology, 120, 110313. doi:10.1016/j.intimp.2023.110313. https://pubmed.ncbi.nlm.nih.gov/37267856/
2. Yuan, Lichan, Zhao, Na, Wang, Junyi, Ben, Jingjing, Ma, Junqing. 2021. Major vault protein (MVP) negatively regulates osteoclastogenesis via calcineurin-NFATc1 pathway inhibition. In Theranostics, 11, 7247-7261. doi:10.7150/thno.58468. https://pubmed.ncbi.nlm.nih.gov/34158848/
3. Wang, Ruobing, Yang, Yan, Zhang, Zhongyin, Ma, Junqing, Yuan, Lichan. 2023. Major vault protein (MVP) suppresses aging- and estrogen deficiency-related bone loss through Fas-mediated apoptosis in osteoclasts. In Cell death & disease, 14, 604. doi:10.1038/s41419-023-05928-4. https://pubmed.ncbi.nlm.nih.gov/37704623/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen