C57BL/6JCya-Ift56em1flox/Cya
Common Name:
Ift56-flox
Product ID:
S-CKO-19188
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ift56-flox
Strain ID
CKOCMP-264134-Ift56-B6J-VB
Gene Name
Product ID
S-CKO-19188
Gene Alias
9330141E21Rik; 9430097H08Rik; Ttc26; hop; hpy
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ift56em1flox/Cya mice (Catalog S-CKO-19188) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000162554
NCBI RefSeq
NM_153600
Target Region
Exon 3~4
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Ift56, also known as TTC26, is an IFTB protein essential for cilia formation and function, which are key regulators of animal development. It is involved in the intraflagellar transport (IFT) process, which is crucial for ciliogenesis, ciliary motility, and signaling [1,2,3,4].
In Ift56hop mouse mutants, which are a kind of KO mouse model, cilia have normal distribution but defective structure, with abnormal positioning and number of ciliary microtubule doublets. These cilia cannot efficiently accumulate Gli proteins, leading to developmental patterning defects in Shh signaling-dependent tissues. Core IFTB proteins like IFT88, IFT81, and IFT27 fail to accumulate normally in Ift56hop cilia, while IFTA components remain unaffected, highlighting Ift56's specific role in maintaining IFTB complex integrity [1]. Additionally, in mammalian cells, KIF17, a homodimeric kinesin-2 protein, binds to the IFT46-IFT56 dimer in the IFT-B complex through its C-terminal sequence for its ciliary entry, suggesting Ift56's role in ciliary protein trafficking [2].
In conclusion, Ift56 plays a distinct and crucial role in maintaining IFTB complex integrity, which is essential for cilia structure and function, and ultimately, animal development. The Ift56hop mouse model has provided valuable insights into the role of Ift56 in developmental patterning defects related to abnormal cilia function, potentially contributing to our understanding of diseases associated with ciliary dysfunction [1].
References:
1. Xin, Daisy, Christopher, Kasey J, Zeng, Lewie, Kong, Yong, Weatherbee, Scott D. 2017. IFT56 regulates vertebrate developmental patterning by maintaining IFTB complex integrity and ciliary microtubule architecture. In Development (Cambridge, England), 144, 1544-1553. doi:10.1242/dev.143255. https://pubmed.ncbi.nlm.nih.gov/28264835/
2. Funabashi, Teruki, Katoh, Yohei, Michisaka, Saki, Sugawa, Maho, Nakayama, Kazuhisa. 2017. Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. In Molecular biology of the cell, 28, 624-633. doi:10.1091/mbc.E16-09-0648. https://pubmed.ncbi.nlm.nih.gov/28077622/
3. Nozaki, Shohei, Katoh, Yohei, Terada, Masaya, Kontani, Kenji, Nakayama, Kazuhisa. 2016. Regulation of ciliary retrograde protein trafficking by the Joubert syndrome proteins ARL13B and INPP5E. In Journal of cell science, 130, 563-576. doi:10.1242/jcs.197004. https://pubmed.ncbi.nlm.nih.gov/27927754/
4. Katoh, Yohei, Terada, Masaya, Nishijima, Yuya, Hamada, Hiroshi, Nakayama, Kazuhisa. 2016. Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex. In The Journal of biological chemistry, 291, 10962-75. doi:10.1074/jbc.M116.713883. https://pubmed.ncbi.nlm.nih.gov/26980730/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen