C57BL/6JCya-Slc35f2em1flox/Cya
Common Name:
Slc35f2-flox
Product ID:
S-CKO-19203
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Slc35f2-flox
Strain ID
CKOCMP-72022-Slc35f2-B6J-VB
Gene Name
Product ID
S-CKO-19203
Gene Alias
1500009K05Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc35f2em1flox/Cya mice (Catalog S-CKO-19203) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000048670
NCBI RefSeq
NM_028060
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Slc35f2, a member of the solute carrier family, belongs to membrane-bound carrier proteins [2,5]. Although its exact physiological function is yet to be fully elucidated, it has been implicated in multiple biological processes related to cancer. It has been associated with pathways such as apoptosis, adhesion, and the p53 signaling pathway [4].
Knockdown of Slc35f2 in bladder cancer cells inhibits their proliferation, migration, and invasion, and promotes apoptosis. In nude mice, Slc35f2 knockdown significantly reduces tumorigenesis, suggesting its role in promoting bladder cancer progression [3]. In pancreatic cancer cells, it has been found that the Slc35f2-SYVN1-TRIM59 axis critically regulates ferroptosis by inhibiting endogenous p53. High expression of Slc35f2 is related to adverse survival outcomes in pancreatic cancer patients [1]. In HeLa cells, depletion of βTrCP1 or APC/CCdh1, which interact with Slc35f2 to promote its ubiquitination and degradation, accumulates Slc35f2 protein and promotes Slc35f2-mediated cell growth, migration, invasion, and colony formation ability, indicating their role in suppressing Slc35f2-mediated carcinogenesis [2,5].
In conclusion, Slc35f2 plays a significant role in cancer-related biological processes, especially in promoting the progression of cancers like bladder and pancreatic cancer. The findings from knockdown experiments in cell lines and in vivo models in nude mice contribute to understanding its role in cancer, suggesting that Slc35f2 could be a potential therapeutic target for treating these cancers [1,2,3,5].
References:
1. Che, Ben, Du, Yunyan, Yuan, Rongfa, Wang, Kai, Chen, Leifeng. 2023. SLC35F2-SYVN1-TRIM59 axis critically regulates ferroptosis of pancreatic cancer cells by inhibiting endogenous p53. In Oncogene, 42, 3260-3273. doi:10.1038/s41388-023-02843-y. https://pubmed.ncbi.nlm.nih.gov/37740007/
2. Colaco, Jencia Carminha, Chandrasekaran, Arun Pandian, Karapurkar, Janardhan Keshav, Kim, Kye-Seong, Ramakrishna, Suresh. 2023. βTrCP1 promotes SLC35F2 protein ubiquitination and inhibits cancer progression in HeLa cells. In Biochemical and biophysical research communications, 682, 27-38. doi:10.1016/j.bbrc.2023.09.095. https://pubmed.ncbi.nlm.nih.gov/37801987/
3. Chen, Mei, Gao, Xin, Huang, Denggao, Cao, Hui, Zhang, Shufang. 2019. Knockdown of SLC35F2 Inhibits the Proliferation and Metastasis of Bladder Cancer Cells. In OncoTargets and therapy, 12, 10771-10786. doi:10.2147/OTT.S229332. https://pubmed.ncbi.nlm.nih.gov/31849485/
4. Zheng, Qingzhu, Li, Mingjie, Qiu, Yingkun, Yang, Jiahao, Cao, Yingping. 2023. Overexpression of SLC35F2 is a potential prognostic biomarker for lung adenocarcinoma. In Heliyon, 10, e23828. doi:10.1016/j.heliyon.2023.e23828. https://pubmed.ncbi.nlm.nih.gov/38187235/
5. Colaco, Jencia Carminha, Chandrasekaran, Arun Pandian, Karapurkar, Janardhan Keshav, Kim, Kye-Seong, Ramakrishna, Suresh. 2023. E3 ubiquitin ligase APC/CCdh1 regulates SLC35F2 protein turnover and inhibits cancer progression in HeLa cells. In Biochimica et biophysica acta. General subjects, 1867, 130454. doi:10.1016/j.bbagen.2023.130454. https://pubmed.ncbi.nlm.nih.gov/37689217/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen