C57BL/6JCya-Pfn2em1flox/Cya
Common Name:
Pfn2-flox
Product ID:
S-CKO-19204
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pfn2-flox
Strain ID
CKOCMP-18645-Pfn2-B6J-VB
Gene Name
Product ID
S-CKO-19204
Gene Alias
Pfn
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pfn2em1flox/Cya mice (Catalog S-CKO-19204) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066882
NCBI RefSeq
NM_019410
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Pfn2, a profilin, binds to globular (G -)actin and regulates actin filament formation, playing a significant role in the actin cytoskeleton, which is crucial for cell shape, division, and intracellular force generation. It is also involved in multiple biological processes and associated with various signaling pathways [1].
In triple negative breast cancer, Pfn2 promotes cell proliferation, migration, invasion, and epithelial-to-mesenchymal transition [2]. In androgen receptor-negative prostate cancer, knockdown of Pfn2 inhibits tumor growth in vivo [3]. In esophageal squamous cell carcinoma, downregulation of Pfn2 inhibits cell invasion and migration and induces an epithelial-mesenchymal transition phenotype [4]. High Pfn2 expression in head and neck squamous cell carcinoma independently predicts poor overall survival [5].
In conclusion, Pfn2 is an important regulator in actin-related biological processes. Its dysregulation is associated with the development and prognosis of multiple cancers. The use of gene knockout or knockdown models in these studies has revealed its role in promoting tumor growth, invasion, and metastasis, suggesting Pfn2 as a potential therapeutic target for these cancer types.
References：
1. Ree, Rasmus, Kind, Laura, Kaziales, Anna, Drazic, Adrian, Arnesen, Thomas. 2020. PFN2 and NAA80 cooperate to efficiently acetylate the N-terminus of actin. In The Journal of biological chemistry, 295, 16713-16731. doi:10.1074/jbc.RA120.015468. https://pubmed.ncbi.nlm.nih.gov/32978259/
2. Ling, Yuwei, Cao, Qi, Liu, Yihan, Kang, Hua, Chen, Zhenwen. 2020. Profilin 2 (PFN2) promotes the proliferation, migration, invasion and epithelial-to-mesenchymal transition of triple negative breast cancer cells. In Breast cancer (Tokyo, Japan), 28, 368-378. doi:10.1007/s12282-020-01169-x. https://pubmed.ncbi.nlm.nih.gov/33047272/
3. Obinata, Daisuke, Funakoshi, Daigo, Takayama, Kenichi, Takahashi, Satoru, Inoue, Satoshi. 2022. OCT1-target neural gene PFN2 promotes tumor growth in androgen receptor-negative prostate cancer. In Scientific reports, 12, 6094. doi:10.1038/s41598-022-10099-x. https://pubmed.ncbi.nlm.nih.gov/35413990/
4. Cui, Xiao-Bin, Zhang, Shu-Mao, Xu, Yue-Xun, Chen, Yun-Zhao, Li, Feng. 2016. PFN2, a novel marker of unfavorable prognosis, is a potential therapeutic target involved in esophageal squamous cell carcinoma. In Journal of translational medicine, 14, 137. doi:10.1186/s12967-016-0884-y. https://pubmed.ncbi.nlm.nih.gov/27188458/
5. Liu, Jiyuan, Wu, Yunlong, Wang, Qizhang, Liao, Xuejuan, Pan, Jian. 2018. Bioinformatic analysis of PFN2 dysregulation and its prognostic value in head and neck squamous carcinoma. In Future oncology (London, England), 14, 449-459. doi:10.2217/fon-2017-0348. https://pubmed.ncbi.nlm.nih.gov/29322815/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen