C57BL/6JCya-Dyrk2em1flox/Cya
Common Name:
Dyrk2-flox
Product ID:
S-CKO-19205
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dyrk2-flox
Strain ID
CKOCMP-69181-Dyrk2-B6J-VB
Gene Name
Product ID
S-CKO-19205
Gene Alias
1810038L18Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dyrk2em1flox/Cya mice (Catalog S-CKO-19205) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000004281
NCBI RefSeq
NM_001014390
Target Region
Exon 3
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
DYRK2, short for dual-specificity tyrosine phosphorylation-regulated kinase 2, is a member of the CMGC kinase family. It plays crucial roles in regulating the cell cycle, apoptosis, DNA repair, and proteostasis [1,2,3,4,5]. DYRK2 phosphorylates key substrates such as p53 at Ser46 to induce apoptosis in response to DNA damage, and is also involved in pathways like the regulation of G1/S transition, epithelial-mesenchymal-transition, and stemness in human cancer cells [3].
In hepatocellular carcinoma, liver-specific Dyrk2 conditional knockout mice and in vivo gene delivery systems revealed that Dyrk2 expression was reduced in tumours, and Dyrk2 gene transfer significantly suppressed carcinogenesis by promoting the degradation of Myc and Hras at the protein level [6]. In colorectal cancer, DYRK2 knockout decreased chemosensitivity to 5-fluorouracil and oxaliplatin in p53 wild-type cells and xenograft mouse models, while overexpression enhanced chemosensitivity through p53-Ser46 phosphorylation [7].
In conclusion, DYRK2 emerges as a key regulator in multiple biological processes, especially in cancer-related events. The use of Dyrk2 knockout and conditional knockout mouse models has significantly contributed to understanding its role in hepatocellular and colorectal cancers, highlighting its potential as a therapeutic target.
References:
1. Tandon, Vasudha, de la Vega, Laureano, Banerjee, Sourav. 2021. Emerging roles of DYRK2 in cancer. In The Journal of biological chemistry, 296, 100233. doi:10.1074/jbc.REV120.015217. https://pubmed.ncbi.nlm.nih.gov/33376136/
2. Mochimaru, Yuta, Yoshida, Kiyotsugu. 2023. Functional Roles of DYRK2 as a Tumor Regulator. In Current issues in molecular biology, 45, 8539-8551. doi:10.3390/cimb45100538. https://pubmed.ncbi.nlm.nih.gov/37886981/
3. Yoshida, Saishu, Yoshida, Kiyotsugu. 2019. Multiple functions of DYRK2 in cancer and tissue development. In FEBS letters, 593, 2953-2965. doi:10.1002/1873-3468.13601. https://pubmed.ncbi.nlm.nih.gov/31505048/
4. Kawamura, Akira, Yoshida, Saishu, Yoshida, Kiyotsugu. 2024. The diverse functions of DYRK2 in response to cellular stress. In Histology and histopathology, 39, 1427-1434. doi:10.14670/HH-18-744. https://pubmed.ncbi.nlm.nih.gov/38656683/
5. Nihira, Naoe Taira, Yoshida, Kiyotsugu. . Engagement of DYRK2 in proper control for cell division. In Cell cycle (Georgetown, Tex.), 14, 802-7. doi:10.1080/15384101.2015.1007751. https://pubmed.ncbi.nlm.nih.gov/25603354/
6. Kamioka, Hiroshi, Yogosawa, Satomi, Oikawa, Tsunekazu, Saruta, Masayuki, Yoshida, Kiyotsugu. 2023. Dyrk2 gene transfer suppresses hepatocarcinogenesis by promoting the degradation of Myc and Hras. In JHEP reports : innovation in hepatology, 5, 100759. doi:10.1016/j.jhepr.2023.100759. https://pubmed.ncbi.nlm.nih.gov/37333975/
7. Takano, Yasuhiro, Yogosawa, Satomi, Imaizumi, Yuta, Eto, Ken, Yoshida, Kiyotsugu. 2023. DYRK2 promotes chemosensitivity via p53-mediated apoptosis after DNA damage in colorectal cancer. In Cancer science, 114, 4558-4570. doi:10.1111/cas.15973. https://pubmed.ncbi.nlm.nih.gov/37776195/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen