C57BL/6JCya-Neat1em1flox/Cya
Common Name:
Neat1-flox
Product ID:
S-CKO-19216
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Neat1-flox
Strain ID
CKOCMP-66961-Neat1-B6J-VA
Gene Name
Product ID
S-CKO-19216
Gene Alias
2310043N10Rik; VINC
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Neat1em1flox/Cya mice (Catalog S-CKO-19216) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000173672
NCBI RefSeq
NR_131212
Target Region
Exon 1
Size of Effective Region
~23.9 kb
Detailed Document
Overview of Gene Research
Neat1, also known as Nuclear paraspeckle assembly transcript 1, is a long noncoding RNA. It is a vital component of paraspeckles, playing a role in their formation and integrity. Neat1 is involved in various biological processes, and its dysregulation is associated with multiple diseases, including different types of cancer, liver-related diseases, type 2 diabetes and its complications, and neurological disorders [4,6-9].
In hepatocellular carcinoma, Neat1 deficiency caused senescence in cultured hepatoma cells and protected against HCC progression in a mouse model, suggesting its role in promoting tumor progression [1]. In atherosclerosis, exercise-mediated down-regulation of Neat1 was shown to mitigate endothelial pyroptosis and delay atherosclerosis, with NEAT1-/-mice used to determine its role [2]. In post-stroke conditions, knockdown of Neat1 in mice significantly alleviated lipid droplet agglomeration and inhibited autophagy, leading to improved cerebral perfusion, reduced brain injury, and increased neurological recovery [3].
In conclusion, Neat1 is crucial in multiple biological processes and disease conditions. Studies using gene knockout (KO) or conditional knockout (CKO) mouse models have revealed its role in promoting tumor progression in hepatocellular carcinoma, its contribution to atherosclerosis development, and its impact on post-stroke outcomes. These findings provide insights into potential therapeutic strategies targeting Neat1 for these diseases.
References:
1. Chen, Danlei, Wang, Jinghao, Li, Yang, Zhang, Pengfei, Liu, Lianxin. . LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A. In Clinical and translational medicine, 13, e1418. doi:10.1002/ctm2.1418. https://pubmed.ncbi.nlm.nih.gov/37752791/
2. Yang, Qingyuan, Chen, Shiliang, Wang, Xingyi, Sun, Yong, Wu, Jian. 2023. Exercise Mitigates Endothelial Pyroptosis and Atherosclerosis by Downregulating NEAT1 Through N6-Methyladenosine Modifications. In Arteriosclerosis, thrombosis, and vascular biology, 43, 910-926. doi:10.1161/ATVBAHA.123.319251. https://pubmed.ncbi.nlm.nih.gov/37078289/
3. Pan, Yongli, Xin, Wenqiang, Wei, Wei, Huttner, Hagen B, Doeppner, Thorsten R. 2024. Knockdown of NEAT1 prevents post-stroke lipid droplet agglomeration in microglia by regulating autophagy. In Cellular and molecular life sciences : CMLS, 81, 30. doi:10.1007/s00018-023-05045-7. https://pubmed.ncbi.nlm.nih.gov/38212456/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen