C57BL/6JCya-Gsta3em1flox/Cya
Common Name:
Gsta3-flox
Product ID:
S-CKO-19225
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gsta3-flox
Strain ID
CKOCMP-14859-Gsta3-B6J-VB
Gene Name
Product ID
S-CKO-19225
Gene Alias
Gst2-3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gsta3em1flox/Cya mice (Catalog S-CKO-19225) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027067
NCBI RefSeq
NM_010356
Target Region
Exon 3~4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Gsta3, glutathione S-transferase alpha 3, is a member of the glutathione S-transferase family with antioxidative function. It is involved in the PI3K-Keap1/Nrf2 pathway, which plays a role in regulating oxidative stress and has significance in various biological processes and disease resistance [1].
In renal interstitial fibrosis, Gsta3 knockdown in NRK-52E cells led to increased production of reactive oxygen species and tubular epithelial-mesenchymal transition, while overexpressing Gsta3 ameliorated these effects, indicating its protective role against tubular EMT in renal fibrosis [2].
In Gsta3 knockout mice, hepatic aflatoxin B1-N(7)-guanine levels were elevated 120-fold compared with wild-type, and the inability to rescue these mice from aflatoxin genotoxicity through the Nrf2 transcriptional program shows that Gsta3 is unilaterally responsible for the detoxication of aflatoxin in mice [3].
In cutaneous squamous cell carcinoma (cSCC), Gsta3 was downregulated. Overexpression of Gsta3 induced a less malignant phenotype, while its silencing led to more malignant phenotypes, and Gsta3 could inhibit cSCC progression through suppressing the TGF-β/Smad and HIF-1α signalings [4].
In conclusion, Gsta3 plays crucial roles in antioxidation, renal interstitial fibrosis prevention, aflatoxin detoxication, and inhibition of cSCC progression. Gene knockout mouse models have significantly contributed to revealing its functions in these disease-related biological processes, providing potential therapeutic targets for relevant diseases.
References：
1. Xiao, Yun, Zhang, Zhiwei, Fu, Yingyu, Cui, Sini, Wu, Jun. 2019. GSTA3 regulates TGF-β1-induced renal interstitial fibrosis in NRK-52E cells as a component of the PI3K-Keap1/Nrf2 pathway. In The Journal of international medical research, 47, 5787-5801. doi:10.1177/0300060519876796. https://pubmed.ncbi.nlm.nih.gov/31617428/
2. Xiao, Yun, Liu, Jishi, Peng, Yu, Zhang, Jian, Tao, Lijian. 2016. GSTA3 Attenuates Renal Interstitial Fibrosis by Inhibiting TGF-Beta-Induced Tubular Epithelial-Mesenchymal Transition and Fibronectin Expression. In PloS one, 11, e0160855. doi:10.1371/journal.pone.0160855. https://pubmed.ncbi.nlm.nih.gov/27602565/
3. Kensler, Kevin H, Slocum, Stephen L, Chartoumpekis, Dionysios V, Kensler, Thomas W, Egner, Patricia A. 2014. Genetic or pharmacologic activation of Nrf2 signaling fails to protect against aflatoxin genotoxicity in hypersensitive GSTA3 knockout mice. In Toxicological sciences : an official journal of the Society of Toxicology, 139, 293-300. doi:10.1093/toxsci/kfu056. https://pubmed.ncbi.nlm.nih.gov/24675090/
4. Li, Weiwei, Qiu, Cheng, Wang, Shujun, Wu, Lijun, Zhao, Tianlan. 2020. Inhibitory effect of glutathione S-transferase A3 in the progression of cutaneous squamous cell carcinoma. In Journal of cosmetic dermatology, 20, 2287-2295. doi:10.1111/jocd.13806. https://pubmed.ncbi.nlm.nih.gov/33089654/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen