C57BL/6JCya-Prelid1em1flox/Cya
Common Name:
Prelid1-flox
Product ID:
S-CKO-19239
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Prelid1-flox
Strain ID
CKOCMP-66494-Prelid1-B6J-VA
Gene Name
Product ID
S-CKO-19239
Gene Alias
2610524G07Rik; Preli
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prelid1em1flox/Cya mice (Catalog S-CKO-19239) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021942
NCBI RefSeq
NM_025596
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
PRELID1 is a gene encoding a protein involved in mitochondrial lipid transport. It belongs to the Ups1/PRELID1 family of lipid transfer proteins, which shuttle phosphatidic acid (PA) across the mitochondrial intermembrane space for conversion to cardiolipin (CL), a crucial phospholipid in mitochondria [3]. This process is essential for maintaining mitochondrial structure and function, and is also associated with unfolded protein response (UPR) and mTORC1 signaling [3].
In cancer research, alternative polyadenylation of PRELID1 mRNA has been shown to enhance its steady-state level and translational efficiency, and is a strong breast-cancer-subtype-dependent predictor of patient clinical outcomes. PRELID1 also regulates stress response and mitochondrial reactive oxygen species (ROS) production in a cell-type-specific manner, suggesting that its expression regulation is a common stress response in different cancer types [2]. In the context of ER stress, PERK signaling promotes the degradation of PRELID1, leading to the accumulation of PA on the outer mitochondrial membrane, which in turn induces mitochondrial elongation [1].
In conclusion, PRELID1 plays a vital role in mitochondrial lipid metabolism, stress response, and cancer-related processes. Studies on PRELID1, including those potentially using gene-knockout models, contribute to understanding the mechanisms underlying mitochondrial function and cancer development, providing insights for potential therapeutic targets.
References:
1. Perea, Valerie, Cole, Christian, Lebeau, Justine, Grotjahn, Danielle A, Wiseman, R Luke. 2023. PERK signaling promotes mitochondrial elongation by remodeling membrane phosphatidic acid. In The EMBO journal, 42, e113908. doi:10.15252/embj.2023113908. https://pubmed.ncbi.nlm.nih.gov/37306086/
2. Gillen, Austin E, Brechbuhl, Heather M, Yamamoto, Tomomi M, Hesselberth, Jay R, Kabos, Peter. 2017. Alternative Polyadenylation of PRELID1 Regulates Mitochondrial ROS Signaling and Cancer Outcomes. In Molecular cancer research : MCR, 15, 1741-1751. doi:10.1158/1541-7786.MCR-17-0010. https://pubmed.ncbi.nlm.nih.gov/28912168/
3. Eiyama, Akinori, Aaltonen, Mari J, Nolte, Hendrik, Tatsuta, Takashi, Langer, Thomas. 2021. Disturbed intramitochondrial phosphatidic acid transport impairs cellular stress signaling. In The Journal of biological chemistry, 296, 100335. doi:10.1016/j.jbc.2021.100335. https://pubmed.ncbi.nlm.nih.gov/33497623/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen