C57BL/6JCya-Npr1em1flox/Cya
Common Name
Npr1-flox
Product ID
S-CKO-19248
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-18160-Npr1-B6J-VB
When using this mouse strain in a publication, please cite “Npr1-flox Mouse (Catalog S-CKO-19248) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Npr1-flox
Strain ID
CKOCMP-18160-Npr1-B6J-VB
Gene Name
Product ID
S-CKO-19248
Gene Alias
GC-A, NPR-A, NPRA, Pndr
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000029540
NCBI RefSeq
NM_008727
Target Region
Exon 2~7
Size of Effective Region
~4.0 kb
Overview of Gene Research
Npr1, or Nonexpressor of pathogenesis-related genes 1, is a key activator of salicylic acid (SA)-mediated immune responses in plants [2]. It plays a central role in systemic acquired resistance, a broad-spectrum disease resistance mechanism, and controls about 90% of SA-dependent transcriptome in Arabidopsis [4]. It is also involved in regulating the crosstalk between SA and other defense and growth hormones, thus being crucial for plant health [2].
In plants, Npr1 promotes cell survival during pathogen effector-triggered immunity (ETI) by forming salicylic acid-induced Npr1 condensates (SINCs). These SINCs target substrates for ubiquitination and degradation, such as EDS1 and specific WRKY transcription factors, through the formation of the Npr1-Cullin 3 E3 ligase complex [1]. Npr1 also functions as a transcriptional co-activator, binding to SA and bridging two TGA transcription factor dimers to form an enhanceosome for transcriptional induction [2,3]. Additionally, Npr1 and its paralogs have been defined as SA receptors, and Npr1's functions in stress protein homeostasis and cell survival are achieved through the formation of distinct nuclear and cytoplasmic biomolecular condensates [2].
In conclusion, Npr1 is a master regulator in plant immunity, with functions in transcriptional regulation, substrate ubiquitination, and cell survival decisions. Understanding Npr1 in plants can potentially be utilized to engineer resistance against biotic and abiotic stresses in crops [2].
References：
1. Zavaliev, Raul, Mohan, Rajinikanth, Chen, Tianyuan, Dong, Xinnian. 2020. Formation of NPR1 Condensates Promotes Cell Survival during the Plant Immune Response. In Cell, 182, 1093-1108.e18. doi:10.1016/j.cell.2020.07.016. https://pubmed.ncbi.nlm.nih.gov/32810437/
2. Zavaliev, Raul, Dong, Xinnian. 2023. NPR1, a key immune regulator for plant survival under biotic and abiotic stresses. In Molecular cell, 84, 131-141. doi:10.1016/j.molcel.2023.11.018. https://pubmed.ncbi.nlm.nih.gov/38103555/
3. Kumar, Shivesh, Zavaliev, Raul, Wu, Qinglin, Dong, Xinnian, Zhou, Pei. 2022. Structural basis of NPR1 in activating plant immunity. In Nature, 605, 561-566. doi:10.1038/s41586-022-04699-w. https://pubmed.ncbi.nlm.nih.gov/35545668/
4. Sun, Yali, Detchemendy, Thomas Ward, Pajerowska-Mukhtar, Karolina Marta, Mukhtar, M Shahid. 2018. NPR1 in JazzSet with Pathogen Effectors. In Trends in plant science, 23, 469-472. doi:10.1016/j.tplants.2018.04.007. https://pubmed.ncbi.nlm.nih.gov/29753632/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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