C57BL/6JCya-Lars1em1flox/Cya
Common Name:
Lars1-flox
Product ID:
S-CKO-19251
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lars1-flox
Strain ID
CKOCMP-107045-Lars1-B6J-VB
Gene Name
Product ID
S-CKO-19251
Gene Alias
2310045K21Rik; 3110009L02Rik; Lars; leuRS
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lars1em1flox/Cya mice (Catalog S-CKO-19251) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000097590
NCBI RefSeq
NM_134137
Target Region
Exon 5~7
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
LARS1, leucyl-tRNA synthetase 1, is an enzyme crucial for protein synthesis, specifically ligating leucine to its cognate tRNA. It also functions as a leucine sensor upstream of the amino acid-sensing pathway, participating in coordinating protein synthesis and autophagy for cell growth [3,7]. LARS1 is involved in multiple pathways, such as mTORC1 regulation in response to glucose and leucine availability [3,4].
In hepatocellular carcinoma (HCC), LARS1 expression is elevated in tumor tissues compared to normal tissues, correlated with various clinicopathological features, and higher expression is linked to worse survival. It may promote HCC through processes like neutral lipid metabolic process and cholesterol metabolism [1].
In ovarian cancer, LARS1 is increased and acts as an oncogene, while the mitochondrial-derived peptide MOTS-c can suppress ovarian cancer progression by promoting LARS1 ubiquitination and degradation [2].
In non-small cell lung cancer, inhibiting LARS1 with BC-LI-0186 in combination with a MEK1/2 inhibitor enhances the anti-tumor effect [5].
Additionally, biallelic variants in LARS1 cause infantile liver failure syndrome type 1 (ILFS1). In a zebrafish model with a LARS1 variant, enhanced autophagy led to hepatic steatosis, suggesting a mechanism for ILFS1-associated hepatic dysfunction [6].
In conclusion, LARS1 is essential for protein synthesis and nutrient-sensing-related cellular processes. Its dysregulation is associated with various cancers and ILFS1. Studies using models like zebrafish with LARS1 variants help reveal its role in disease-related biological processes, providing insights into potential therapeutic strategies for these diseases.
References:
1. Fan, Longfei, Qin, Zhongqiang, Wu, Di, Tan, Yulin, Tan, Yi. 2024. LARS1 is a Prognostic Biomarker and Exhibits a Correlation with Immune Infiltrates in Hepatocellular Carcinoma. In International journal of general medicine, 17, 2203-2221. doi:10.2147/IJGM.S457577. https://pubmed.ncbi.nlm.nih.gov/38774724/
2. Yin, Yadong, Li, Yujie, Ma, Boyi, Yang, Hong, Li, Jibin. 2024. Mitochondrial-Derived Peptide MOTS-c Suppresses Ovarian Cancer Progression by Attenuating USP7-Mediated LARS1 Deubiquitination. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2405620. doi:10.1002/advs.202405620. https://pubmed.ncbi.nlm.nih.gov/39321430/
3. Yoon, Ina, Nam, Miso, Kim, Hoi Kyoung, Kim, Jong Hyun, Kim, Sunghoon. 2019. Glucose-dependent control of leucine metabolism by leucyl-tRNA synthetase 1. In Science (New York, N.Y.), 367, 205-210. doi:10.1126/science.aau2753. https://pubmed.ncbi.nlm.nih.gov/31780625/
4. Kim, Kibum, Yoo, Hee Chan, Kim, Byung Gyu, Kim, Sunghoon, Han, Jung Min. 2022. O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine. In Nature communications, 13, 2904. doi:10.1038/s41467-022-30696-8. https://pubmed.ncbi.nlm.nih.gov/35614056/
5. Lee, Sang Hoon, Kim, Eun Young, Han, Jung Min, Han, Gyoonhee, Chang, Yoon Soo. 2023. Combination of the LARS1 Inhibitor, BC-LI-0186 with a MEK1/2 Inhibitor Enhances the Anti-Tumor Effect in Non-Small Cell Lung Cancer. In Cancer research and treatment, 55, 851-864. doi:10.4143/crt.2022.1527. https://pubmed.ncbi.nlm.nih.gov/36960627/
6. Inoue, Masanori, Sebastian, Wulan Apridita, Sonoda, Shota, Hanada, Toshikatsu, Ihara, Kenji. 2024. Biallelic variants in LARS1 induce steatosis in developing zebrafish liver via enhanced autophagy. In Orphanet journal of rare diseases, 19, 219. doi:10.1186/s13023-024-03226-6. https://pubmed.ncbi.nlm.nih.gov/38807157/
7. Yu, Ya Chun, Han, Jung Min, Kim, Sunghoon. 2020. Aminoacyl-tRNA synthetases and amino acid signaling. In Biochimica et biophysica acta. Molecular cell research, 1868, 118889. doi:10.1016/j.bbamcr.2020.118889. https://pubmed.ncbi.nlm.nih.gov/33091505/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen