C57BL/6JCya-Ppt2em1flox/Cya
Common Name:
Ppt2-flox
Product ID:
S-CKO-19257
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ppt2-flox
Strain ID
CKOCMP-54397-Ppt2-B6J-VB
Gene Name
Product ID
S-CKO-19257
Gene Alias
0610007M19Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppt2em1flox/Cya mice (Catalog S-CKO-19257) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000171376
NCBI RefSeq
NM_019441
Target Region
Exon 3~6
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Check the content of the review
Ppt2, or palmitoyl protein thioesterase 2, is a lysosomal thioesterase. It is highly associated with metabolism [1,2]. In fungi like Candida albicans, Ppt2 (phosphopantetheinyl transferase) is essential for growth as it transfers the phosphopantetheinyl group of coenzyme A to the acyl carrier protein Acp1 in mitochondria for lipoic acid synthesis, which is crucial for fungal respiration [3,5].
In mouse models, disruption of PPT2 causes an unusual lysosomal storage disorder with neurovisceral features. PPT2-deficient mice show a neurodegenerative phenotype with spasticity and ataxia by 15 months. There are also visceral manifestations such as infiltration of the bone marrow by autofluorescent macrophages, splenomegaly due to extramedullary hematopoiesis, and lipofuscin pigment storage in the exocrine cells of the pancreas [4]. In addition, both PPT1 and PPT2 knockout mice develop spasticity, with motor abnormalities leading to death in PPT1 knockout mice by 10 months, while most PPT2 knockout mice are alive at 12 months. Autofluorescent storage material is present in the brains of both strains [6].
In conclusion, Ppt2 is important in metabolism and its deficiency in mouse models leads to a neurodegenerative disorder with visceral features. These mouse models have revealed the role of Ppt2 in neurodegeneration and related pathological processes, highlighting its potential significance in understanding certain human diseases [4,6].
References:
1. Xu, Hui, Zhang, Yan, Xie, Zhen, Zhao, Bei-Bei, Hua, Tian. 2024. Investigating PPT2's role in ovarian cancer prognosis and immunotherapy outcomes. In Journal of ovarian research, 17, 198. doi:10.1186/s13048-024-01527-9. https://pubmed.ncbi.nlm.nih.gov/39394143/
2. Yuan, ChangFei, Xiong, ZhiYong, Shi, Jian, Chen, Ke, Zhang, XiaoPing. 2020. Overexpression of PPT2 Represses the Clear Cell Renal Cell Carcinoma Progression by Reducing Epithelial-to-mesenchymal Transition. In Journal of Cancer, 11, 1151-1161. doi:10.7150/jca.36477. https://pubmed.ncbi.nlm.nih.gov/31956361/
3. Meng, Ling-Ning, Liu, Jin-Yan, Wang, Yu-Ting, Ni, Shuai-Shuai, Xiang, Ming-Jie. 2020. The discovery of potential phosphopantetheinyl transferase Ppt2 inhibitors against drug-resistant Candida albicans. In Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 51, 1665-1672. doi:10.1007/s42770-020-00318-w. https://pubmed.ncbi.nlm.nih.gov/32557281/
4. Gupta, Praveena, Soyombo, Abigail A, Shelton, John M, Richardson, James A, Hofmann, Sandra L. 2003. Disruption of PPT2 in mice causes an unusual lysosomal storage disorder with neurovisceral features. In Proceedings of the National Academy of Sciences of the United States of America, 100, 12325-30. doi:. https://pubmed.ncbi.nlm.nih.gov/14528005/
5. Dobb, Katharine S, Kaye, Sarah J, Beckmann, Nicola, Birch, Mike, Oliver, Jason D. 2015. Characterisation of the Candida albicans Phosphopantetheinyl Transferase Ppt2 as a Potential Antifungal Drug Target. In PloS one, 10, e0143770. doi:10.1371/journal.pone.0143770. https://pubmed.ncbi.nlm.nih.gov/26606674/
6. Gupta, P, Soyombo, A A, Atashband, A, Hammer, R E, Hofmann, S L. . Disruption of PPT1 or PPT2 causes neuronal ceroid lipofuscinosis in knockout mice. In Proceedings of the National Academy of Sciences of the United States of America, 98, 13566-71. doi:. https://pubmed.ncbi.nlm.nih.gov/11717424/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen