C57BL/6JCya-Samd7em1flox/Cya
Common Name:
Samd7-flox
Product ID:
S-CKO-19265
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Samd7-flox
Strain ID
CKOCMP-75953-Samd7-B6J-VB
Gene Name
Product ID
S-CKO-19265
Gene Alias
4930597A01Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Samd7em1flox/Cya mice (Catalog S-CKO-19265) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108262
NCBI RefSeq
NM_029489
Target Region
Exon 4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Samd7, short for sterile alpha motif domain containing 7, is a component of the Polycomb repressive complex 1 (PRC1). It plays a crucial role in transcriptional regulation, inhibiting the transcription of many genes, especially those activated by the transcription factor Cone-Rod Homeobox (CRX). It is essential for retinal function and is involved in pathways related to photoreceptor cell identity establishment and gene expression regulation in the retina [1,3,5].
In Samd7-null mutant mice, non-rod genes like S-opsin are ectopically expressed in rod photoreceptor cells, leading to rod photoreceptor cell dysfunction [3]. In zebrafish, loss of Samd7 results in red cones transforming into hybrid red/ultraviolet (UV) cones, absence of green cones, an approximate doubling of blue cones, and a significant reduction in the number of rods [2]. Also, in the Samd7/11 double-knock-out (DKO) mouse retina, photoreceptor outer segments are shortened, and more retinal genes are up-regulated compared to Samd7 -/- alone, indicating partial functional redundancy between Samd7 and Samd11 [4].
In conclusion, Samd7 is vital for maintaining appropriate gene expression patterns in rods and cone subtypes, thus ensuring normal retinal function. Studies using gene-knockout mouse models have revealed its role in preventing non-rod gene expression in rod photoreceptor cells and in establishing correct photoreceptor cell identities. These findings contribute significantly to understanding autosomal-recessive macular dystrophy and other retinal-related diseases [1,3].
References:
1. Bauwens, Miriam, Celik, Elifnaz, Zur, Dinah, De Baere, Elfride, Ben-Yosef, Tamar. 2024. Mutations in SAMD7 cause autosomal-recessive macular dystrophy with or without cone dysfunction. In American journal of human genetics, 111, 393-402. doi:10.1016/j.ajhg.2024.01.001. https://pubmed.ncbi.nlm.nih.gov/38272031/
2. Volkov, Leo I, Ogawa, Yohey, Somjee, Ramiz, Kefalov, Vladimir J, Corbo, Joseph C. 2024. Samd7 represses short-wavelength cone genes to preserve long-wavelength cone and rod photoreceptor identity. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2402121121. doi:10.1073/pnas.2402121121. https://pubmed.ncbi.nlm.nih.gov/39531499/
3. Omori, Yoshihiro, Kubo, Shun, Kon, Tetsuo, Nakagawa, Atsushi, Furukawa, Takahisa. 2017. Samd7 is a cell type-specific PRC1 component essential for establishing retinal rod photoreceptor identity. In Proceedings of the National Academy of Sciences of the United States of America, 114, E8264-E8273. doi:10.1073/pnas.1707021114. https://pubmed.ncbi.nlm.nih.gov/28900001/
4. Kubo, Shun, Yamamoto, Haruka, Kajimura, Naoko, Chaya, Taro, Furukawa, Takahisa. 2021. Functional analysis of Samd11, a retinal photoreceptor PRC1 component, in establishing rod photoreceptor identity. In Scientific reports, 11, 4180. doi:10.1038/s41598-021-83781-1. https://pubmed.ncbi.nlm.nih.gov/33603070/
5. Hlawatsch, Julia, Karlstetter, Marcus, Aslanidis, Alexander, Böck, Julia, Langmann, Thomas. 2013. Sterile alpha motif containing 7 (samd7) is a novel crx-regulated transcriptional repressor in the retina. In PloS one, 8, e60633. doi:10.1371/journal.pone.0060633. https://pubmed.ncbi.nlm.nih.gov/23565263/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen