C57BL/6JCya-Fbh1em1flox/Cya
Common Name:
Fbh1-flox
Product ID:
S-CKO-19267
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fbh1-flox
Strain ID
CKOCMP-50755-Fbh1-B6J-VB
Gene Name
Product ID
S-CKO-19267
Gene Alias
Fbx18; Fbxo18
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fbh1em1flox/Cya mice (Catalog S-CKO-19267) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000071564
NCBI RefSeq
NM_015792
Target Region
Exon 4~6
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Fbh1, or F-box DNA helicase 1, is a member of the UvrD family of DNA helicases [2,5]. It plays a crucial role in the cell's response to replicative stress [2,5]. FBH1 is involved in pathways such as homologous recombination regulation, as it inhibits homologous recombination and catalyzes fork regression when recruited to stalled DNA replication forks by PCNA [2]. It also promotes double-strand break formation and signaling in response to prolonged replication stress, which can initiate apoptosis [3].
FBH1-deficient cells show a reduction in ssDNA and double-strand break signaling when treated with WEE1 inhibitors, indicating its requirement for the induction of replication stress response [1,4]. However, FBH1-deficiency sensitizes cells to WEE1 inhibition by increasing mitotic catastrophe [1,4]. Also, depletion of FBH1 promotes UV-mediated transformation of human melanocytes, suggesting its inactivation contributes to oncogenic transformation [5]. In addition, RAD51 promotes replication stress resistance in FBH1-deficient cells, and depletion of UBC13, which promotes RAD51-dependent template switching, sensitizes FBH1-deficient cells to replication stress, indicating FBH1 regulates template switching to promote replication stress sensitivity [3].
In conclusion, FBH1 is essential for regulating the cell's response to replicative stress, influencing processes like homologous recombination, replication fork handling, and double-strand break signaling. The study of FBH1-deficient models has revealed its significance in cancer-related processes such as oncogenic transformation and response to anti-cancer therapies like WEE1 inhibition, providing insights into potential cancer treatment strategies.
References:
1. Jennings, Lucy, Walters, Heather Andrews, Mason, Jennifer M. 2023. FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.05.15.540841. https://pubmed.ncbi.nlm.nih.gov/37292855/
2. Liu, Jiuyang, Chaves-Arquero, Belén, Wei, Pengcheng, Blanco, Francisco J, Kutateladze, Tatiana G. 2023. Molecular insight into the PCNA-binding mode of FBH1. In Structure (London, England : 1993), 31, 511-517.e3. doi:10.1016/j.str.2023.03.004. https://pubmed.ncbi.nlm.nih.gov/36990095/
3. Hawks, Alexandra L, Bergmann, Amy, McCraw, Tyler J, Mason, Jennifer M. 2023. UBC13-mediated template switching promotes replication stress resistance in FBH1-deficient cells. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.09.04.556280. https://pubmed.ncbi.nlm.nih.gov/37732269/
4. Jennings, Lucy, Walters, Heather Andrews, McCraw, Tyler J, Turner, Joshua L, Mason, Jennifer M. 2023. FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe. In DNA repair, 133, 103611. doi:10.1016/j.dnarep.2023.103611. https://pubmed.ncbi.nlm.nih.gov/38103522/
5. Jeong, Yeon-Tae, Cermak, Lukas, Guijarro, Maria V, Hernando, Eva, Pagano, Michele. 2013. FBH1 protects melanocytes from transformation and is deregulated in melanomas. In Cell cycle (Georgetown, Tex.), 12, 1128-32. doi:10.4161/cc.24165. https://pubmed.ncbi.nlm.nih.gov/23466708/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen