C57BL/6JCya-Fbh1em1flox/Cya
Common Name
Fbh1-flox
Product ID
S-CKO-19267
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-50755-Fbh1-B6J-VB
When using this mouse strain in a publication, please cite “Fbh1-flox Mouse (Catalog S-CKO-19267) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Fbh1-flox
Strain ID
CKOCMP-50755-Fbh1-B6J-VB
Gene Name
Product ID
S-CKO-19267
Gene Alias
Fbx18, Fbxo18
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000071564
NCBI RefSeq
NM_015792
Target Region
Exon 4~6
Size of Effective Region
~2.5 kb
Overview of Gene Research
Fbh1, or F-box DNA helicase 1, is a member of the UvrD family of DNA helicases [2,5]. It plays a crucial role in the cell's response to replicative stress [2,5]. FBH1 is involved in pathways such as homologous recombination regulation, as it inhibits homologous recombination and catalyzes fork regression when recruited to stalled DNA replication forks by PCNA [2]. It also promotes double-strand break formation and signaling in response to prolonged replication stress, which can initiate apoptosis [3].
FBH1-deficient cells show a reduction in ssDNA and double-strand break signaling when treated with WEE1 inhibitors, indicating its requirement for the induction of replication stress response [1,4]. However, FBH1-deficiency sensitizes cells to WEE1 inhibition by increasing mitotic catastrophe [1,4]. Also, depletion of FBH1 promotes UV-mediated transformation of human melanocytes, suggesting its inactivation contributes to oncogenic transformation [5]. In addition, RAD51 promotes replication stress resistance in FBH1-deficient cells, and depletion of UBC13, which promotes RAD51-dependent template switching, sensitizes FBH1-deficient cells to replication stress, indicating FBH1 regulates template switching to promote replication stress sensitivity [3].
In conclusion, FBH1 is essential for regulating the cell's response to replicative stress, influencing processes like homologous recombination, replication fork handling, and double-strand break signaling. The study of FBH1-deficient models has revealed its significance in cancer-related processes such as oncogenic transformation and response to anti-cancer therapies like WEE1 inhibition, providing insights into potential cancer treatment strategies.
References：
1. Jennings, Lucy, Walters, Heather Andrews, Mason, Jennifer M. 2023. FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.05.15.540841. https://pubmed.ncbi.nlm.nih.gov/37292855/
2. Liu, Jiuyang, Chaves-Arquero, Belén, Wei, Pengcheng, Blanco, Francisco J, Kutateladze, Tatiana G. 2023. Molecular insight into the PCNA-binding mode of FBH1. In Structure (London, England : 1993), 31, 511-517.e3. doi:10.1016/j.str.2023.03.004. https://pubmed.ncbi.nlm.nih.gov/36990095/
3. Hawks, Alexandra L, Bergmann, Amy, McCraw, Tyler J, Mason, Jennifer M. 2023. UBC13-mediated template switching promotes replication stress resistance in FBH1-deficient cells. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.09.04.556280. https://pubmed.ncbi.nlm.nih.gov/37732269/
4. Jennings, Lucy, Walters, Heather Andrews, McCraw, Tyler J, Turner, Joshua L, Mason, Jennifer M. 2023. FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe. In DNA repair, 133, 103611. doi:10.1016/j.dnarep.2023.103611. https://pubmed.ncbi.nlm.nih.gov/38103522/
5. Jeong, Yeon-Tae, Cermak, Lukas, Guijarro, Maria V, Hernando, Eva, Pagano, Michele. 2013. FBH1 protects melanocytes from transformation and is deregulated in melanomas. In Cell cycle (Georgetown, Tex.), 12, 1128-32. doi:10.4161/cc.24165. https://pubmed.ncbi.nlm.nih.gov/23466708/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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