C57BL/6JCya-Faf2em1flox/Cya
Common Name:
Faf2-flox
Product ID:
S-CKO-19268
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Faf2-flox
Strain ID
CKOCMP-76577-Faf2-B6J-VB
Gene Name
Product ID
S-CKO-19268
Gene Alias
2210404D11Rik; Ubxd8; mKIAA0887
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Faf2em1flox/Cya mice (Catalog S-CKO-19268) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000126071
NCBI RefSeq
NM_178397
Target Region
Exon 4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Faf2, also known as Fas-associated factor 2, UBXD8 or ETEA, is a ubiquitin ligase adaptor protein. It is involved in the ubiquitin-mediated degradation of misfolded proteins in the endoplasmic reticulum and is associated with multiple biological pathways such as lipid metabolism and peroxisomal homeostasis [1,2]. Genetic models, like zebrafish and mouse models, are valuable for studying its functions [2,3].
In zebrafish larvae, depletion of faf2 using CRISPR-Cas9 genome editing significantly increased the effects of ethanol and high-fat diet (HFD) toxicity, leading to increased hepatic steatosis and hepatic neutrophil recruitment. Ethanol or HFD exposure also significantly altered the expression of genes associated with ethanol and lipid metabolism in faf2-depleted larvae [3].
In mice, knocking down Faf2 using AAV-delivered shRNA mitigated alcohol-induced hepatic steatosis. Transcriptomic analysis showed that differentially expressed genes were enriched in lipid metabolism regulation pathways, and Faf2 knockdown affected the expression of SREBP1 target genes and the FOXO3-SIRT6-PCSK9 pathway, as well as adipose triglyceride lipase-related proteins [2].
In conclusion, Faf2 plays a crucial role in lipid metabolism, especially in processes related to alcohol-induced liver steatosis. The use of gene-knockout models in zebrafish and mice has revealed its significance in regulating lipid-related biological processes and provided insights into its potential as a target for treating alcohol-associated liver disease.
References：
1. Koyano, Fumika, Yamano, Koji, Hoshina, Tomoyuki, Tanaka, Keiji, Matsuda, Noriyuki. 2024. AAA+ ATPase chaperone p97/VCPFAF2 governs basal pexophagy. In Nature communications, 15, 9347. doi:10.1038/s41467-024-53558-x. https://pubmed.ncbi.nlm.nih.gov/39472561/
2. Huda, Nazmul, Kusumanchi, Praveen, Jiang, Yanchao, Ma, Jing, Yang, Zhihong. 2025. Silencing FAF2 mitigates alcohol-induced hepatic steatosis by modulating lipolysis and PCSK9 pathway. In Hepatology communications, 9, . doi:10.1097/HC9.0000000000000641. https://pubmed.ncbi.nlm.nih.gov/39969435/
3. Shihana, Fathima, Cholan, Pradeep Manuneedhi, Fraser, Stuart, Oehlers, Stefan H, Seth, Devanshi. 2023. Investigating the role of lipid genes in liver disease using fatty liver models of alcohol and high fat in zebrafish (Danio rerio). In Liver international : official journal of the International Association for the Study of the Liver, 43, 2455-2468. doi:10.1111/liv.15716. https://pubmed.ncbi.nlm.nih.gov/37650211/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen