C57BL/6JCya-Mcm8em1flox/Cya
Common Name:
Mcm8-flox
Product ID:
S-CKO-19269
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mcm8-flox
Strain ID
CKOCMP-66634-Mcm8-B6J-VB
Gene Name
Product ID
S-CKO-19269
Gene Alias
5730432L01Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mcm8em1flox/Cya mice (Catalog S-CKO-19269) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028831
NCBI RefSeq
NM_001291054
Target Region
Exon 11~12
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Mcm8, or Minichromosome Maintenance 8 Homologous Recombination Repair Factor, is a helicase involved in multiple DNA-related processes. It plays a crucial role in DNA replication (initiation), meiosis, homologous recombination, and mismatch repair. Mcm8 is also associated with pathways like mitophagy and R-loop resolution, which are essential for maintaining genome stability and cell function [3].
Disabling Mcm8 in mice led to proliferation defects of primordial germ cells (PGCs) and ultimately impaired fertility. This indicates its significance in PGC development and reproductive reserve establishment. Loss of Mcm8 also led to R-loop accumulation by reducing the retention of helicases DDX5 and DHX9 at R-loops, thus inducing genome instability [1]. In a mouse model of Kawasaki disease, Mcm8-deficient mice developed more severe coronary artery vasculopathy, suggesting its role in protecting vascular health [2].
In conclusion, Mcm8 is essential for maintaining genome integrity, with key functions in PGC development and vascular health as revealed by mouse models. Its role in R-loop resolution and mitophagy provides insights into the molecular mechanisms underlying reproductive aging and Kawasaki disease, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Wen, Canxin, Cao, Lili, Wang, Shuhan, Yang, Yajuan, Qin, Yingying. 2024. MCM8 interacts with DDX5 to promote R-loop resolution. In The EMBO journal, 43, 3044-3071. doi:10.1038/s44318-024-00134-0. https://pubmed.ncbi.nlm.nih.gov/38858601/
2. Lin, Meng, Xian, Huifang, Chen, Zhanghua, Gu, Xiaoqiong, Zhang, Yuxia. 2023. MCM8-mediated mitophagy protects vascular health in response to nitric oxide signaling in a mouse model of Kawasaki disease. In Nature cardiovascular research, 2, 778-792. doi:10.1038/s44161-023-00314-x. https://pubmed.ncbi.nlm.nih.gov/39195969/
3. Helderman, Noah Cornelis, Terlouw, Diantha, Bonjoch, Laia, Goldberg, Yael, Nielsen, Maartje. 2023. Molecular functions of MCM8 and MCM9 and their associated pathologies. In iScience, 26, 106737. doi:10.1016/j.isci.2023.106737. https://pubmed.ncbi.nlm.nih.gov/37378315/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen