C57BL/6JCya-Mcm9em1flox/Cya
Common Name:
Mcm9-flox
Product ID:
S-CKO-19271
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mcm9-flox
Strain ID
CKOCMP-71567-Mcm9-B6J-VB
Gene Name
Product ID
S-CKO-19271
Gene Alias
9030408O17Rik; Gm235; Mcmdc1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mcm9em1flox/Cya mice (Catalog S-CKO-19271) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000237608
NCBI RefSeq
NM_027830
Target Region
Exon 4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Mcm9, also known as Minichromosome Maintenance 9 Homologous Recombination Repair Factor, is a crucial protein in multiple DNA-related processes. It is involved in DNA replication (specifically in the assembly of prereplication complexes where it binds Cdt1 and is required for loading the MCM2-7 helicase onto DNA replication origins) [5], meiosis, homologous recombination, and DNA mismatch repair [1,3]. These functions are vital for maintaining genomic integrity, which is essential for normal cellular function and development.
In knockout mouse models, mutations in related genes like Hrob, which recruits the MCM8-MCM9 helicase to sites of DNA damage, lead to infertility due to germ cell depletion and meiotic arrest, suggesting Mcm9's role in meiosis and germ cell development [6]. In humans, MCM9 mutations are associated with primary ovarian insufficiency, Sertoli cell-only syndrome, and early-onset cancer [2,4]. For example, homozygous pathogenic MCM9 variants in humans can cause ovarian dysgenesis in females and non-obstructive azoospermia in males [4].
In conclusion, Mcm9 is essential for DNA-related processes such as replication, meiosis, and DNA repair. Model-based research, including gene knockout studies in mice and human genetic studies, has revealed its significance in diseases like infertility and early-onset cancer. Understanding Mcm9's functions provides insights into the mechanisms underlying these diseases, potentially leading to better diagnostic and treatment strategies.
References:
1. Helderman, Noah Cornelis, Terlouw, Diantha, Bonjoch, Laia, Goldberg, Yael, Nielsen, Maartje. 2023. Molecular functions of MCM8 and MCM9 and their associated pathologies. In iScience, 26, 106737. doi:10.1016/j.isci.2023.106737. https://pubmed.ncbi.nlm.nih.gov/37378315/
2. Goldberg, Yael, Aleme, Ola, Peled-Perets, Lilach, Nielsen, Maartje, Shalev, Stavit A. 2021. MCM9 is associated with germline predisposition to early-onset cancer-clinical evidence. In NPJ genomic medicine, 6, 78. doi:10.1038/s41525-021-00242-4. https://pubmed.ncbi.nlm.nih.gov/34556653/
3. Traver, Sabine, Coulombe, Philippe, Peiffer, Isabelle, Latreille, Daniel, Méchali, Marcel. 2015. MCM9 Is Required for Mammalian DNA Mismatch Repair. In Molecular cell, 59, 831-9. doi:10.1016/j.molcel.2015.07.010. https://pubmed.ncbi.nlm.nih.gov/26300262/
4. Potorac, Iulia, Laterre, Marie, Malaise, Olivier, Pétrossians, Patrick, Pintiaux, Axelle. 2023. The Role of MCM9 in the Etiology of Sertoli Cell-Only Syndrome and Premature Ovarian Insufficiency. In Journal of clinical medicine, 12, . doi:10.3390/jcm12030990. https://pubmed.ncbi.nlm.nih.gov/36769638/
5. Lutzmann, Malik, Méchali, Marcel. . MCM9 binds Cdt1 and is required for the assembly of prereplication complexes. In Molecular cell, 31, 190-200. doi:10.1016/j.molcel.2008.07.001. https://pubmed.ncbi.nlm.nih.gov/18657502/
6. Hustedt, Nicole, Saito, Yuichiro, Zimmermann, Michal, Jurisicova, Andrea, Durocher, Daniel. 2019. Control of homologous recombination by the HROB-MCM8-MCM9 pathway. In Genes & development, 33, 1397-1415. doi:10.1101/gad.329508.119. https://pubmed.ncbi.nlm.nih.gov/31467087/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen