C57BL/6JCya-Bap1em1flox/Cya
Common Name:
Bap1-flox
Product ID:
S-CKO-19296
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Bap1-flox
Strain ID
CKOCMP-104416-Bap1-B6J-VB
Gene Name
Product ID
S-CKO-19296
Gene Alias
2300006C11Rik; mKIAA0272; uch-x4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bap1em1flox/Cya mice (Catalog S-CKO-19296) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022458
NCBI RefSeq
NM_027088
Target Region
Exon 4~5
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
BAP1, short for BRCA1-Associated Protein 1, is a ubiquitin carboxy-terminal hydrolase functioning as a tumor suppressor. It utilizes its deubiquitinating activity to regulate multiple processes such as DNA damage repair, cell cycle control, chromatin modification, programmed cell death, and the immune response [1,3,4,6]. It is also involved in metabolic regulation of ferroptosis, an important non-apoptotic form of cell death [2].
In normal human cholangiocyte organoids, CRISPR-Cas9-induced BAP1 loss-of-function led to loss of epithelial characteristics and increased motility, with restoration of BAP1's catalytic activity in the nucleus rescuing these changes. In a genetic background of human liver organoids engineered with four common cholangiocarcinoma mutations, BAP1 loss resulted in acquisition of malignant features upon xenotransplantation [5].
In conclusion, BAP1 is crucial for maintaining normal cellular functions through its regulation of diverse biological processes. The use of genetic models like CRISPR-engineered organoids has revealed its significance in tumor suppression, especially in cancers such as cholangiocarcinoma. Germline BAP1 mutations are associated with a high risk of developing multiple cancers, defining the "BAP1 cancer syndrome" [3,4,7].
References:
1. Louie, Bryan H, Kurzrock, Razelle. 2020. BAP1: Not just a BRCA1-associated protein. In Cancer treatment reviews, 90, 102091. doi:10.1016/j.ctrv.2020.102091. https://pubmed.ncbi.nlm.nih.gov/32877777/
2. Zhang, Yilei, Shi, Jiejun, Liu, Xiaoguang, Li, Wei, Gan, Boyi. 2018. BAP1 links metabolic regulation of ferroptosis to tumour suppression. In Nature cell biology, 20, 1181-1192. doi:10.1038/s41556-018-0178-0. https://pubmed.ncbi.nlm.nih.gov/30202049/
3. Masclef, Louis, Ahmed, Oumaima, Estavoyer, Benjamin, Nijnik, Anastasia, Affar, El Bachir. 2021. Roles and mechanisms of BAP1 deubiquitinase in tumor suppression. In Cell death and differentiation, 28, 606-625. doi:10.1038/s41418-020-00709-4. https://pubmed.ncbi.nlm.nih.gov/33462414/
4. Carbone, Michele, Harbour, J William, Brugarolas, James, Yang, Haining, Gaudino, Giovanni. 2020. Biological Mechanisms and Clinical Significance of BAP1 Mutations in Human Cancer. In Cancer discovery, 10, 1103-1120. doi:10.1158/2159-8290.CD-19-1220. https://pubmed.ncbi.nlm.nih.gov/32690542/
5. Artegiani, Benedetta, van Voorthuijsen, Lisa, Lindeboom, Rik G H, Vermeulen, Michiel, Clevers, Hans. 2019. Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids. In Cell stem cell, 24, 927-943.e6. doi:10.1016/j.stem.2019.04.017. https://pubmed.ncbi.nlm.nih.gov/31130514/
6. Kwon, Jongbum, Lee, Daye, Lee, Shin-Ai. 2023. BAP1 as a guardian of genome stability: implications in human cancer. In Experimental & molecular medicine, 55, 745-754. doi:10.1038/s12276-023-00979-1. https://pubmed.ncbi.nlm.nih.gov/37009801/
7. Lalloo, Fiona, Kulkarni, Anju, Chau, Cindy, Tischkowitz, Marc, Hanson, Helen. 2023. Clinical practice guidelines for the diagnosis and surveillance of BAP1 tumour predisposition syndrome. In European journal of human genetics : EJHG, 31, 1261-1269. doi:10.1038/s41431-023-01448-z. https://pubmed.ncbi.nlm.nih.gov/37607989/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen