C57BL/6JCya-Myh4em1flox/Cya
Common Name:
Myh4-flox
Product ID:
S-CKO-19313
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Myh4-flox
Strain ID
CKOCMP-17884-Myh4-B6J-VB
Gene Name
Product ID
S-CKO-19313
Gene Alias
MHC2B; MM; MYH-2B; Minimsc; Minmus; MyHC-IIb; Myhsf
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Myh4em1flox/Cya mice (Catalog S-CKO-19313) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018632
NCBI RefSeq
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Target Region
Exon 5~10
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Myh4, encoding myosin heavy chain IIB (MyHC-IIB), is crucial for muscle function. MyHC-IIB expression is associated with muscle hypertrophic growth [2]. Muscle fibers in adult rodent skeletal muscles are classified into 4 subtypes based on myosin heavy chain proteins, with Myh4 corresponding to type IIB fibers [3].
In Myh4 null mice, postnatal muscle growth is affected. The gastrocnemius muscle loses up to 50% of its fibers between 2-4 weeks of age, and to compensate, type I, IIA, and IIX(D) fibers increase in prevalence and size. Stimulating the oxidative program in muscle only partially protects from fiber loss. Traditional means of stimulating muscle hypertrophy, like breeding with myostatin null mice or feeding clenbuterol, have little impact on Myh4 -/- mice, suggesting existing fibers have maximized their compensatory capacity [1].
In conclusion, Myh4 is essential for muscle fiber maintenance, transition, and hypertrophy during postnatal muscle growth. The study of Myh4 null mice provides insights into the role of Myh4 in muscle development and offers new avenues for attenuating fast-twitch fiber loss in aged or diseased muscles [1].
References:
1. Zeng, Caiyun, Shi, Hao, Kirkpatrick, Laila T, Grant, Alan L, Gerrard, David E. 2022. Driving an Oxidative Phenotype Protects Myh4 Null Mice From Myofiber Loss During Postnatal Growth. In Frontiers in physiology, 12, 785151. doi:10.3389/fphys.2021.785151. https://pubmed.ncbi.nlm.nih.gov/35283757/
2. Brearley, Madelaine C, Loczenski-Brown, David M, Loughna, Paul T, Parr, Tim, Brameld, John M. 2021. Response of the porcine MYH4-promoter and MYH4-expressing myotubes to known anabolic and catabolic agents in vitro. In Biochemistry and biophysics reports, 25, 100924. doi:10.1016/j.bbrep.2021.100924. https://pubmed.ncbi.nlm.nih.gov/33614996/
3. Wang, Chao, Yue, Feng, Kuang, Shihuan. . Muscle Histology Characterization Using H&E Staining and Muscle Fiber Type Classification Using Immunofluorescence Staining. In Bio-protocol, 7, . doi:10.21769/BioProtoc.2279. https://pubmed.ncbi.nlm.nih.gov/28752107/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen